{"title":"A Perylene-Based Bright Red-Emitting Probe for Wash-Free Cell Plasma Membrane Imaging Via Excimer Formation.","authors":"Mingshu Wu, Yongling Zheng, Ziwen Yan, Junying Ma, Siqi Fan, Enju Wang","doi":"10.1007/s10895-025-04315-w","DOIUrl":null,"url":null,"abstract":"<p><p>Excimer emission exhibits red-shifted, broadened, and large Stokes-shifted characteristics compared to monomeric emission, making it advantageous for biological imaging. In this work, a perylene-based amphiphilic fluorophore (DAVP) was developed for wash-free cell plasma membrane imaging. DAVP readily forms excimer, emitting intense red fluorescence. In water, it shows very weak fluorescence, but its emission intensity significantly increases upon molecular aggregation induced by either increasing concentration or adding THF to aqueous solutions. Notably, DAVP exhibits enhanced fluorescence in cationic and anionic surfactant micelles compared to its aggregated states. Leveraging these properties, DAVP serves as a membrane-specific imaging agent with several distinct advantages: bright red emission, a large Stokes shift, high water solubility, low cytotoxicity, rapid staining, and wash-free capability.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Fluorescence","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1007/s10895-025-04315-w","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
Abstract
Excimer emission exhibits red-shifted, broadened, and large Stokes-shifted characteristics compared to monomeric emission, making it advantageous for biological imaging. In this work, a perylene-based amphiphilic fluorophore (DAVP) was developed for wash-free cell plasma membrane imaging. DAVP readily forms excimer, emitting intense red fluorescence. In water, it shows very weak fluorescence, but its emission intensity significantly increases upon molecular aggregation induced by either increasing concentration or adding THF to aqueous solutions. Notably, DAVP exhibits enhanced fluorescence in cationic and anionic surfactant micelles compared to its aggregated states. Leveraging these properties, DAVP serves as a membrane-specific imaging agent with several distinct advantages: bright red emission, a large Stokes shift, high water solubility, low cytotoxicity, rapid staining, and wash-free capability.
期刊介绍：
Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.
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