Promising protective potential of MiR-103a-3p against polystyrene microplastic neurotoxicity in rats.

IF 3.6 Q2 TOXICOLOGY
Frontiers in toxicology Pub Date : 2025-04-01 eCollection Date: 2025-01-01 DOI:10.3389/ftox.2025.1560980
Leila Mohammadi, Tourandokht Baluchnejadmojarad, Mina Goudarzi, Vahid Khodashenas, Roya Khoshravesh, Mehrdad Roghani
{"title":"Promising protective potential of MiR-103a-3p against polystyrene microplastic neurotoxicity in rats.","authors":"Leila Mohammadi, Tourandokht Baluchnejadmojarad, Mina Goudarzi, Vahid Khodashenas, Roya Khoshravesh, Mehrdad Roghani","doi":"10.3389/ftox.2025.1560980","DOIUrl":null,"url":null,"abstract":"<p><p><b>Introduction:</b> Microplastics are ubiquitous environmental pollutants with potential neurotoxic effects that can impair learning and memory. MicroRNAs are essential regulators of a number of physiological and pathological processes, but detailed information on the impact of miRNAs on the neurotoxic effects of microplastics is lacking. <b>Methods:</b> In the present study, polystyrene microplastics (PS-MPs) were administered orally and miR-103a-3p was injected intracerebroventricularly as a treatment for PS-MPs-induced neurotoxicity. <b>Results and Discussion:</b> Performance in the novel object discrimination Y-maze and Barnes maze tests indicated that miR-103a-3p mitigates the deleterious effects of PS-MPs on learning and memory. Oxidative stress, pyroptosis, apoptosis and inflammation induced by PS-MPs were modulated after miR- 103a-3p injection by reducing malondialdehyde, protein carbonyl, nitrite, caspase 3, caspase 1, TNFα, and NLRP3 levels in hippocampal tissue. Our results also showed that miR-103a-3p can reverse the impact of PS-MPs on astrocytic reaction and SIRT1 and BDNF levels. MiR-103a-3p alleviated PS-MPs-induced endoplasmic reticulum (ER) stress through reducing the levels of PERK, CHOP and GRP78. These findings imply that miR-103a-3p exerts a neuroprotective influence against cognitive deficits induced by exposure to PS-MPs. This is achieved by reducing inflammation, oxidative stress, apoptosis and endoplasmic reticulum stress.</p>","PeriodicalId":73111,"journal":{"name":"Frontiers in toxicology","volume":"7 ","pages":"1560980"},"PeriodicalIF":3.6000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11996803/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in toxicology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3389/ftox.2025.1560980","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"TOXICOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Introduction: Microplastics are ubiquitous environmental pollutants with potential neurotoxic effects that can impair learning and memory. MicroRNAs are essential regulators of a number of physiological and pathological processes, but detailed information on the impact of miRNAs on the neurotoxic effects of microplastics is lacking. Methods: In the present study, polystyrene microplastics (PS-MPs) were administered orally and miR-103a-3p was injected intracerebroventricularly as a treatment for PS-MPs-induced neurotoxicity. Results and Discussion: Performance in the novel object discrimination Y-maze and Barnes maze tests indicated that miR-103a-3p mitigates the deleterious effects of PS-MPs on learning and memory. Oxidative stress, pyroptosis, apoptosis and inflammation induced by PS-MPs were modulated after miR- 103a-3p injection by reducing malondialdehyde, protein carbonyl, nitrite, caspase 3, caspase 1, TNFα, and NLRP3 levels in hippocampal tissue. Our results also showed that miR-103a-3p can reverse the impact of PS-MPs on astrocytic reaction and SIRT1 and BDNF levels. MiR-103a-3p alleviated PS-MPs-induced endoplasmic reticulum (ER) stress through reducing the levels of PERK, CHOP and GRP78. These findings imply that miR-103a-3p exerts a neuroprotective influence against cognitive deficits induced by exposure to PS-MPs. This is achieved by reducing inflammation, oxidative stress, apoptosis and endoplasmic reticulum stress.

MiR-103a-3p对大鼠聚苯乙烯微塑料神经毒性的保护潜力。
微塑料是普遍存在的环境污染物,具有潜在的神经毒性作用,可以损害学习和记忆。microrna是许多生理和病理过程的重要调节因子,但关于microrna对微塑料神经毒性影响的详细信息尚缺乏。方法:在本研究中,通过口服聚苯乙烯微塑料(PS-MPs)和脑室内注射miR-103a-3p来治疗PS-MPs诱导的神经毒性。结果和讨论:在新型物体辨别y迷宫和Barnes迷宫测试中的表现表明,miR-103a-3p减轻了PS-MPs对学习和记忆的有害影响。miR- 103a-3p注射后,通过降低海马组织丙二醛、蛋白羰基、亚硝酸盐、caspase 3、caspase 1、TNFα和NLRP3水平,调节PS-MPs诱导的氧化应激、焦亡、细胞凋亡和炎症反应。我们的研究结果还表明,miR-103a-3p可以逆转PS-MPs对星形细胞反应以及SIRT1和BDNF水平的影响。MiR-103a-3p通过降低PERK、CHOP和GRP78水平减轻ps - mps诱导的内质网(ER)应激。这些发现表明,miR-103a-3p对暴露于PS-MPs诱导的认知缺陷具有神经保护作用。这是通过减少炎症、氧化应激、细胞凋亡和内质网应激来实现的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
3.80
自引率
0.00%
发文量
0
审稿时长
13 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信