Genomic perspectives on NDM Salmonella Typhi, and a case report from India.

IF 5.4 2区医学 Q1 INFECTIOUS DISEASES

Infection Pub Date : 2025-05-12 DOI:10.1007/s15010-025-02546-4

Shruthi Vasanthaiah, Pritibala Takey, Prasanna Kumar Selvam, Supraja Mohan, Ravi Kiran, Shabnam Roohi, Karthick Vasudevan

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引用次数: 0

Abstract

Background: Carbapenem resistance in Enterobacterales is a growing public health concern, primarily driven by carbapenemase enzymes such as OXA-48, VIM, NDM, and IMP. Among these, New Delhi Metallo-β-lactamase (NDM) has disseminated widely across various Enterobacterales species, including Salmonella Typhi, though reports remain rare.

Case presentation: We report an 11-year-old boy from Bangalore with a 10-day history of high-grade fever, chills, rigors, and cough. Laboratory investigations revealed elevated CRP, normal CBC, and microcytic hypochromic anemia. A respiratory panel detected Human Rhinovirus/ Enterovirus. Blood cultures grew non-lactose fermenting gram-negative bacilli, identified as Salmonella spp. via Vitek ID/AST. The isolate exhibited resistance to ampicillin, ciprofloxacin, ceftriaxone, tetracycline, and meropenem but remained susceptible to azithromycin, chloramphenicol, and Co-trimoxazole. Serotyping confirmed the serotype as Salmonella Typhi. Whole-genome sequencing (Illumina) revealed blaNDM-5 and aac(6')-Ia, InCX3 plasmid, and the fluoroquinolone resistance-associated gyrAS83Y mutation. Phylogenetic analysis placed the isolate (IOB-SWH-01) within a cluster of recently sequenced S. Typhi strains from India belonging to the H58 haplotype.

Discussion and conclusion: To date, NDM-producing S. Typhi has been reported only once, from Pakistan. This is the first documented case in India. The presence of blaNDM-5 in S. Typhi poses a serious clinical and public health threat, given its multidrug-resistant nature and potential for interspecies transmission. Continued genomic surveillance is crucial to monitor its spread and guide treatment strategies.

查看原文本刊更多论文

NDM伤寒沙门氏菌的基因组观点，以及来自印度的病例报告。

背景：肠杆菌中碳青霉烯耐药是一个日益增长的公共卫生问题，主要是由碳青霉烯酶如OXA-48、VIM、NDM和IMP驱动的。其中，新德里金属β-内酰胺酶（NDM）在各种肠杆菌中广泛传播，包括伤寒沙门氏菌，尽管报道仍然很少。病例介绍：我们报告一名来自班加罗尔的11岁男孩，他有10天的高热、寒战、僵硬和咳嗽史。实验室调查显示CRP升高，CBC正常，小细胞性低色性贫血。呼吸道小组检测到人类鼻病毒/肠道病毒。血液培养培养出非乳糖发酵革兰氏阴性杆菌，经Vitek ID/AST鉴定为沙门氏菌。该分离株对氨苄西林、环丙沙星、头孢曲松、四环素和美罗培南耐药，但对阿奇霉素、氯霉素和复方新诺明敏感。血清分型证实血清型为伤寒沙门氏菌。全基因组测序（Illumina）显示blaNDM-5和aac（6’）-Ia， InCX3质粒，以及氟喹诺酮类药物耐药相关的gyrAS83Y突变。系统发育分析将分离物（IOB-SWH-01）置于最近测序的印度伤寒沙门氏菌群中，属于H58单倍型。讨论和结论：迄今为止，产生ndm的伤寒沙门氏菌仅报道过一次，来自巴基斯坦。这是印度第一例有记录的病例。考虑到blaNDM-5的多重耐药性质和种间传播的可能性，它在伤寒沙门氏菌中的存在构成了严重的临床和公共卫生威胁。持续的基因组监测对于监测其传播和指导治疗策略至关重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Infection 医学-传染病学

CiteScore

12.50

自引率

1.30%

发文量

224

审稿时长

6-12 weeks

期刊介绍： Infection is a journal dedicated to serving as a global forum for the presentation and discussion of clinically relevant information on infectious diseases. Its primary goal is to engage readers and contributors from various regions around the world in the exchange of knowledge about the etiology, pathogenesis, diagnosis, and treatment of infectious diseases, both in outpatient and inpatient settings. The journal covers a wide range of topics, including: Etiology: The study of the causes of infectious diseases. Pathogenesis: The process by which an infectious agent causes disease. Diagnosis: The methods and techniques used to identify infectious diseases. Treatment: The medical interventions and strategies employed to treat infectious diseases. Public Health: Issues of local, regional, or international significance related to infectious diseases, including prevention, control, and management strategies. Hospital Epidemiology: The study of the spread of infectious diseases within healthcare settings and the measures to prevent nosocomial infections. In addition to these, Infection also includes a specialized "Images" section, which focuses on high-quality visual content, such as images, photographs, and microscopic slides, accompanied by brief abstracts. This section is designed to highlight the clinical and diagnostic value of visual aids in the field of infectious diseases, as many conditions present with characteristic clinical signs that can be diagnosed through inspection, and imaging and microscopy are crucial for accurate diagnosis. The journal's comprehensive approach ensures that it remains a valuable resource for healthcare professionals and researchers in the field of infectious diseases.