Xiaojing Qian, Ying Chen, Song Wang, Jian Chen, He Wang, Cheng Hu, Shan Lin
{"title":"Qualitative, Quantitative Analysis and Pharmacokinetic Study of Potentially Active Components of Huangxueyishen Decoction Based on UPLC-MS/MS.","authors":"Xiaojing Qian, Ying Chen, Song Wang, Jian Chen, He Wang, Cheng Hu, Shan Lin","doi":"10.1093/chromsci/bmaf022","DOIUrl":null,"url":null,"abstract":"<p><p>Huangxueyishen decoction (HXYS) is proven effective in treating chronic renal failure in clinical practice. The study aims to identify and analyze the chemical constituents of HXYS decoction, and to quantitatively analyze and perform the study of the pharmacokinetics of its main active ingredients. The Ultra-Performance Liquid Chromatography-Linear Trap Quadrupole-Orbitrap Mass Spectrometry (UPLC-LTQ-Orbitrap-MS) system was used to qualitatively analyze the main components of HXYS. Among these compounds, UPLC-QTRAP-MS/MS was applied to further perform the simultaneous quantification of selected important constituents, and pharmacokinetic studies were performed on eight blood-entry components. A total of 87 compounds were tentatively identified, including 26 flavonoids and flavonoid glycosides, 9 phthalides, 9 phenylpropanoids, 9 lignans and 30 other compounds. Two rapid, sensitive and reliable UPLC-MS/MS method have been successfully developed for the simultaneous determination of 11 constituents and 8 components in the plasma of rats at different time points after HXYS administration. Tmax of the eight components was 0.88-4.00 h, t1/2 was 0.65 ~ 4.58 h and AUC(0-∞) ranged from 145.88 ~ 1785.63 μg·h/L. In this study, the chemical components of HXYS were comprehensively characterized, and the active components were further screened in combination with network pharmacology, which predicted and verified the chemical material basis of HXYS and laid the foundation for analyzing its bioactive substances.</p>","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":"63 4","pages":""},"PeriodicalIF":1.5000,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of chromatographic science","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1093/chromsci/bmaf022","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
Abstract
Huangxueyishen decoction (HXYS) is proven effective in treating chronic renal failure in clinical practice. The study aims to identify and analyze the chemical constituents of HXYS decoction, and to quantitatively analyze and perform the study of the pharmacokinetics of its main active ingredients. The Ultra-Performance Liquid Chromatography-Linear Trap Quadrupole-Orbitrap Mass Spectrometry (UPLC-LTQ-Orbitrap-MS) system was used to qualitatively analyze the main components of HXYS. Among these compounds, UPLC-QTRAP-MS/MS was applied to further perform the simultaneous quantification of selected important constituents, and pharmacokinetic studies were performed on eight blood-entry components. A total of 87 compounds were tentatively identified, including 26 flavonoids and flavonoid glycosides, 9 phthalides, 9 phenylpropanoids, 9 lignans and 30 other compounds. Two rapid, sensitive and reliable UPLC-MS/MS method have been successfully developed for the simultaneous determination of 11 constituents and 8 components in the plasma of rats at different time points after HXYS administration. Tmax of the eight components was 0.88-4.00 h, t1/2 was 0.65 ~ 4.58 h and AUC(0-∞) ranged from 145.88 ~ 1785.63 μg·h/L. In this study, the chemical components of HXYS were comprehensively characterized, and the active components were further screened in combination with network pharmacology, which predicted and verified the chemical material basis of HXYS and laid the foundation for analyzing its bioactive substances.
期刊介绍：
The Journal of Chromatographic Science is devoted to the dissemination of information concerning all methods of chromatographic analysis. The standard manuscript is a description of recent original research that covers any or all phases of a specific separation problem, principle, or method. Manuscripts which have a high degree of novelty and fundamental significance to the field of separation science are particularly encouraged. It is expected the authors will clearly state in the Introduction how their method compares in some markedly new and improved way to previous published related methods. Analytical performance characteristics of new methods including sensitivity, tested limits of detection or quantification, accuracy, precision, and specificity should be provided. Manuscripts which describe a straightforward extension of a known analytical method or an application to a previously analyzed and/or uncomplicated sample matrix will not normally be reviewed favorably. Manuscripts in which mass spectrometry is the dominant analytical method and chromatography is of marked secondary importance may be declined.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
