Kumar Ebineshan, Aparna Srikantam, Michael Sukumar Pallapati
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引用次数: 0
Abstract
Background and objectives: Staphylococcus aureus (S. aureus) is one of the predominant biofilm producing pathogen in leprosy foot ulcer (LFU). The objective of this study was to identify the transcriptome profile through Next Generation Sequencing (NGS) approach in mature biofilm of leprosy foot ulcer isolate of S. aureus.
Materials and methods: A cross-sectional study was conducted from July 2019 to May 2022 and a total of twenty-seven S. aureus isolates were collected from the foot ulcers of leprosy patients. All S. aureus isolates were screened for biofilm formation in vitro. Initially, two potential biofilm producing isolates and two planktonic cells were selected for transcriptome comparison.
Results: With reference to transcriptome profile, out of 2,842 genes, 2,688 genes in mature biofilm and 2,685 genes in planktonic cells were expressed. Among them, forty-five differentially expressed genes with 32 and 13 genes showing up and down regulation respectively were obtained.
Conclusion: The research emphasizes the need for continued exploration into the mechanisms of biofilm formation by S. aureus, particularly in the context of leprosy foot ulcers. Understanding these pathways not only aids in grasping the complexity of chronic infections but also paves the way for innovative therapeutic approaches aimed at mitigating biofilm-related complications in clinical settings.
期刊介绍：
The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.
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