Effect of Acotiamide on Detrusor Underactivity Induced Through Bilateral Pelvic Nerve Crush Injury in Rats.

IF 1.8 3区医学 Q3 UROLOGY & NEPHROLOGY

International Neurourology Journal Pub Date : 2025-03-01 Epub Date: 2025-03-31 DOI:10.5213/inj.2448344.172

Ei-Ichiro Takaoka, Kenji Nagahama, Jun Kamei, Toru Sugihara, Satoshi Ando, Tetsuya Fujimura, Naoki Yoshimura

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引用次数: 0

Abstract

Purpose: To investigate the effectiveness of acotiamide on lower urinary tract dysfunction by using a rat model of neurogenic underactive bladder induced through pelvic nerve crush (PNC) injury.

Methods: Bilateral PNC injuries were performed on 8-week-old female Sprague-Dawley rats (PNC group); the sham surgery group was used as control (control group). Two weeks after surgery, awake cystometrography (CMG) was performed, and acotiamide (10 or 100 mg/kg) was subcutaneously administered to the control and PNC groups. Subsequently, CMG parameter values obtained before and after treatment were compared.

Results: In baseline CMG, compared to control group, PNC group revealed statistically significant elevations in the intercontraction intervals (ICIs), number of nonvoiding contractions, baseline pressure, threshold pressure, bladder capacity, voided volumes, and postvoid residual. However, contraction amplitudes and voiding efficiency were significantly decreased. In the control group, compared with the baseline values, 10-mg/kg acotiamide resulted in statistically significant elevations in contraction amplitudes. Treatment with 100-mg/kg acotiamide led to statistically significant elevations in contraction amplitudes and decreases in ICI and bladder volume. In the PNC group, there were no statistically significant changes noted in CMG parameters after treatment with 10-mg/kg acotiamide (n=6). Compared with the baseline values, the administration of 100-mg/kg acotiamide significantly decreased ICI (1,025±186 seconds vs. 578±161 seconds; P=0.012), bladder capacity (1,841±323 µL vs. 871±174 µL, respectively; P=0.0059) and postvoid residual (223±46 µL vs. 44±22 µL, respectively; P=0.023), and increased contraction amplitudes (22.09±1.76 cm H2O vs. 43.84±6.87 cm H2O, respectively; P=0.012) and voiding efficiency (0.87±0.02 vs. 0.94±0.03, respectively; P=0.029).

Conclusion: Acotiamide showed effectiveness in the treatment of underactive bladder, possibly through activation of bladder afferent and detrusor activities.

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阿哥替胺对双侧骨盆神经挤压损伤致逼尿肌活动不足的影响。

目的：采用大鼠盆腔神经挤压伤（PNC）致神经源性膀胱活动不足模型，探讨阿哥替胺对下尿路功能障碍的影响。方法：8周龄雌性sd - dawley大鼠双侧PNC损伤（PNC组）；假手术组为对照组（control group）。术后2周行清醒膀胱造影（CMG），对照组和PNC组分别皮下注射10或100 mg/kg的阿哥替胺。然后比较治疗前后的CMG参数值。结果：在基线CMG中，与对照组相比，PNC组在收缩间期（ICIs）、非排尿收缩次数、基线压力、阈值压力、膀胱容量、排尿容积和排尿后残留方面均有统计学意义的升高。然而，收缩幅度和排尿效率明显下降。在对照组中，与基线值相比，10mg /kg阿甲硫胺导致收缩幅度有统计学意义的升高。用100 mg/kg阿哥替胺治疗可导致收缩幅度升高、ICI和膀胱体积减少，具有统计学意义。在PNC组中，10 mg/kg阿柯替胺治疗后CMG参数无统计学意义变化（n=6）。与基线值相比，给药100 mg/kg可显著降低ICI（1025±186秒vs. 578±161秒）；P=0.012)，膀胱容量分别为1841±323µL和871±174µL；P=0.0059)和空隙后残留（223±46µL vs. 44±22µL）；P=0.023)，收缩幅度增大（22.09±1.76 cm H2O vs 43.84±6.87 cm H2O）；P=0.012)和排尿效率（分别为0.87±0.02∶0.94±0.03）；P = 0.029)。结论：阿哥替胺可能通过激活膀胱传入神经和逼尿肌活动来治疗膀胱活动不足。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Neurourology Journal UROLOGY & NEPHROLOGY-

CiteScore

4.40

自引率

21.70%

发文量

审稿时长

4 weeks

期刊介绍： The International Neurourology Journal (Int Neurourol J, INJ) is a quarterly international journal that publishes high-quality research papers that provide the most significant and promising achievements in the fields of clinical neurourology and fundamental science. Specifically, fundamental science includes the most influential research papers from all fields of science and technology, revolutionizing what physicians and researchers practicing the art of neurourology worldwide know. Thus, we welcome valuable basic research articles to introduce cutting-edge translational research of fundamental sciences to clinical neurourology. In the editorials, urologists will present their perspectives on these articles. The original mission statement of the INJ was published on October 12, 1997. INJ provides authors a fast review of their work and makes a decision in an average of three to four weeks of receiving submissions. If accepted, articles are posted online in fully citable form. Supplementary issues will be published interim to quarterlies, as necessary, to fully allow berth to accept and publish relevant articles.