{"title":"Novel cultivation techniques for water lily (Nymphaea micrantha Guill. & Perr) production based on in vitro technology.","authors":"Fei Lin, Yong Kang, Arwa Abdulkreem Al-Huqail, Dikhnah Alshehri, Yamei Li, Yuhua Guo, Guangsui Yang, Fenling Tang, Junmei Yin, Zheli Ding, Mamdouh A Eissa","doi":"10.1186/s13007-025-01377-6","DOIUrl":null,"url":null,"abstract":"<p><p>Water lily (Nymphaea micrantha Guill. & Perr) is an aquatic plant that is well known for its nutritional value and medicinal uses. The lack of adequate information regarding propagation and farming techniques has led to the low utilization of this valuable plant. To address the knowledge gap in the use of water lily rhizomes as explants in tissue culture, in this study, the processes of sterilization, induction, proliferation, and rooting in water lily tissue culture are examined. Laboratory experiments were conducted to determine the best methods, materials, and concentrations to develop an ideal method for producing water lily plants. Disinfection with 75% C<sub>2</sub>H<sub>5</sub>OH for 2 min + 0.1% HgCl<sub>2</sub> for 15 min produced the best results, with a contamination rate of 30% and a browning rate of 25%, according to the data. The results indicated that indole-3-butyric acid (IBA) is the optimal plant growth regulator for the induction of water lily rhizomes. Medium containing 3 mg L<sup>-1</sup> of 6-BA was most suitable for the induction of water lily adventitious shoots, with an induction rate of up to 80% and a yield of 2 to 8 shoots. The induction rate of water lily adventitious shoots approached 80% in medium supplemented with 3 mg L<sup>-1</sup> 6-benzylaminopurine (6-BA). The best medium for inducing root development contained IBA at a concentration of 0.5 mg L<sup>-1</sup>, which resulted in rapid root elongation. The best tissue culture techniques identified in the present study were successful in growing full water lily plants with good and vigorous growth from tuberous rhizomes to flowering plants. This early success in water lily tissue culture technology provides crucial technical assistance for ex vitro preservation and water lily seedling growth. This study offers a workable answer to one of the most significant obstacles preventing the spread of the use of water lily culture by outlining a good technique that results in robust and healthy seedlings, which helps lower the cost of cultivation.</p>","PeriodicalId":20100,"journal":{"name":"Plant Methods","volume":"21 1","pages":"56"},"PeriodicalIF":4.4000,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12042527/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Methods","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1186/s13007-025-01377-6","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
Abstract
Water lily (Nymphaea micrantha Guill. & Perr) is an aquatic plant that is well known for its nutritional value and medicinal uses. The lack of adequate information regarding propagation and farming techniques has led to the low utilization of this valuable plant. To address the knowledge gap in the use of water lily rhizomes as explants in tissue culture, in this study, the processes of sterilization, induction, proliferation, and rooting in water lily tissue culture are examined. Laboratory experiments were conducted to determine the best methods, materials, and concentrations to develop an ideal method for producing water lily plants. Disinfection with 75% C2H5OH for 2 min + 0.1% HgCl2 for 15 min produced the best results, with a contamination rate of 30% and a browning rate of 25%, according to the data. The results indicated that indole-3-butyric acid (IBA) is the optimal plant growth regulator for the induction of water lily rhizomes. Medium containing 3 mg L-1 of 6-BA was most suitable for the induction of water lily adventitious shoots, with an induction rate of up to 80% and a yield of 2 to 8 shoots. The induction rate of water lily adventitious shoots approached 80% in medium supplemented with 3 mg L-1 6-benzylaminopurine (6-BA). The best medium for inducing root development contained IBA at a concentration of 0.5 mg L-1, which resulted in rapid root elongation. The best tissue culture techniques identified in the present study were successful in growing full water lily plants with good and vigorous growth from tuberous rhizomes to flowering plants. This early success in water lily tissue culture technology provides crucial technical assistance for ex vitro preservation and water lily seedling growth. This study offers a workable answer to one of the most significant obstacles preventing the spread of the use of water lily culture by outlining a good technique that results in robust and healthy seedlings, which helps lower the cost of cultivation.
期刊介绍：
Plant Methods is an open access, peer-reviewed, online journal for the plant research community that encompasses all aspects of technological innovation in the plant sciences.
There is no doubt that we have entered an exciting new era in plant biology. The completion of the Arabidopsis genome sequence, and the rapid progress being made in other plant genomics projects are providing unparalleled opportunities for progress in all areas of plant science. Nevertheless, enormous challenges lie ahead if we are to understand the function of every gene in the genome, and how the individual parts work together to make the whole organism. Achieving these goals will require an unprecedented collaborative effort, combining high-throughput, system-wide technologies with more focused approaches that integrate traditional disciplines such as cell biology, biochemistry and molecular genetics.
Technological innovation is probably the most important catalyst for progress in any scientific discipline. Plant Methods’ goal is to stimulate the development and adoption of new and improved techniques and research tools and, where appropriate, to promote consistency of methodologies for better integration of data from different laboratories.
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