{"title":"Isolation and characterization of novel microsatellite markers for the invasive ant pest Dolichoderus thoracicus (Hymenoptera: Formicidae).","authors":"Joanne Tzu-Chia Chen, Feng-Chuan Hsu, Shu-Ping Tseng","doi":"10.1093/jisesa/ieaf030","DOIUrl":null,"url":null,"abstract":"<p><p>The black cocoa ant, Dolichoderus thoracicus (Smith 1860), has become a major pest in Taiwan over the past decade. Although a cryptic invasion involving the coexistence of native (mtClade I) and nonnative (mtClade II) lineages within the same species has been reported, key biological features of this species, including reproductive mode, colony structure, dispersal patterns, and population dynamics, remain poorly understood, partially due to the lack of available genetic markers. Accordingly, the present study developed and characterized 20 polymorphic microsatellite markers for D. thoracicus. The number of alleles per locus ranged from 2 to 11 (average = 6). The results suggest a significant level of genetic differentiation between the mtClade I and mtClade II populations. These markers will facilitate studies on gene flow, breeding structure, and colony organization, aiding pest management efforts.</p>","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 2","pages":""},"PeriodicalIF":2.1000,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11997965/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Insect Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1093/jisesa/ieaf030","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The black cocoa ant, Dolichoderus thoracicus (Smith 1860), has become a major pest in Taiwan over the past decade. Although a cryptic invasion involving the coexistence of native (mtClade I) and nonnative (mtClade II) lineages within the same species has been reported, key biological features of this species, including reproductive mode, colony structure, dispersal patterns, and population dynamics, remain poorly understood, partially due to the lack of available genetic markers. Accordingly, the present study developed and characterized 20 polymorphic microsatellite markers for D. thoracicus. The number of alleles per locus ranged from 2 to 11 (average = 6). The results suggest a significant level of genetic differentiation between the mtClade I and mtClade II populations. These markers will facilitate studies on gene flow, breeding structure, and colony organization, aiding pest management efforts.
期刊介绍：
The Journal of Insect Science was founded with support from the University of Arizona library in 2001 by Dr. Henry Hagedorn, who served as editor-in-chief until his death in January 2014. The Entomological Society of America was very pleased to add the Journal of Insect Science to its publishing portfolio in 2014. The fully open access journal publishes papers in all aspects of the biology of insects and other arthropods from the molecular to the ecological, and their agricultural and medical impact.
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