[The effect of vitrectomy on changes in molecular biomarker levels of the vitreous cavity in the early postoperative period in proliferative diabetic retinopathy].

Q3 Medicine

Vestnik oftalmologii Pub Date : 2025-01-01 DOI:10.17116/oftalma202514102130

Yu Yusef, D V Petrachkov, K V Baryshev, A G Matyushchenko

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引用次数: 0

Abstract

Determining the levels of molecular biomarkers detected in the vitreous body in various pathological conditions (including diabetic retinopathy), allows for a better understanding of their pathogenesis and optimization of treatment approaches. The multiplex assay is a modern alternative to enzyme-linked immunosorbent assay (ELISA), enabling the simultaneous analysis of a large number of biomarkers.

Purpose: This study was performed to identify changes in the levels of vitreous biomarkers in patients with proliferative diabetic retinopathy (PDR) in the early postoperative period following 25-gauge vitrectomy.

Material and methods: The study included 24 patients (24 eyes) with type 2 diabetes mellitus complicated by PDR requiring surgical intervention for tractional retinal detachment, subtotal or total vitreous hemorrhage, or tractional diabetic macular edema (DME). Vitreous samples were collected from all patients at the first stage of vitrectomy and one month after surgery.

Results: One month after surgery, the level of the pro-inflammatory cytokine vascular endothelial growth factor (VEGF) decreased from 203.8±228.0 to 113±95 pg/ml; however, the difference was not statistically significant (p=0.307). A significant reduction was observed in the concentrations of the following pro-inflammatory biomarkers: bFGF, CTACK, Eotaxin, HGF, IL-1β, -1α, -4, -12 (p40), -16, -18, IL-2Rα, IP-10, MIG, SCF, SCGF-β, SDF-1α, MIF, and M-CSF.

Conclusion: The main effect of vitrectomy on pro-inflammatory cytokines is reduction of their concentration, which confirms the buffering role of the vitreous body in the accumulation of molecular biomarkers in diabetic retinopathy.

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[玻璃体切除术对增殖性糖尿病视网膜病变术后早期玻璃体腔分子生物标志物水平变化的影响]。

确定在各种病理条件下（包括糖尿病视网膜病变）玻璃体中检测到的分子生物标志物水平，可以更好地了解其发病机制和优化治疗方法。多重测定法是酶联免疫吸附测定法（ELISA）的现代替代方法，可以同时分析大量生物标志物。目的：本研究旨在确定增殖性糖尿病视网膜病变（PDR）患者术后早期玻璃体生物标志物水平的变化。材料和方法：本研究纳入24例（24眼）2型糖尿病合并PDR，因牵引性视网膜脱离、玻璃体次全或全出血或牵引性糖尿病性黄斑水肿（DME）需要手术干预的患者。所有患者在玻璃体切除术第一阶段和术后1个月采集玻璃体标本。结果：术后1个月，促炎细胞因子血管内皮生长因子（VEGF）水平由203.8±228.0降至113±95 pg/ml；但差异无统计学意义（p=0.307）。以下促炎生物标志物的浓度显著降低：bFGF、CTACK、Eotaxin、HGF、IL-1β、-1α、-4、-12 （p40）、-16、-18、IL-2Rα、IP-10、MIG、SCF、SCGF-β、SDF-1α、MIF和M-CSF。结论：玻璃体切除术对促炎细胞因子的主要影响是降低其浓度，证实了玻璃体在糖尿病视网膜病变分子生物标志物积累中的缓冲作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Vestnik oftalmologii Medicine-Ophthalmology

CiteScore

0.80

自引率

0.00%

发文量

129

期刊介绍： The journal publishes materials on the diagnosis and treatment of eye diseases, hygiene of vision, prevention of ophthalmic affections, history of Russian ophthalmology, organization of ophthalmological aid to the population, as well as the problems of special equipment. Original scientific articles and surveys on urgent problems of theory and practice of Russian and foreign ophthalmology are published. The journal contains book reviews on ophthalmology, information on the activities of ophthalmologists" scientific societies, chronicle of congresses and conferences.The journal is intended for ophthalmologists and scientific workers dealing with clinical problems of diseases of the eye and physiology of vision.