Identifying potential tear biomarkers in premature infants with retinopathy of prematurity based on proteome and transcriptome analysis.

IF 2.4 3区医学 Q2 OPHTHALMOLOGY

Graefe’s Archive for Clinical and Experimental Ophthalmology Pub Date : 2025-05-09 DOI:10.1007/s00417-025-06838-1

Dongting Wu, Zixin Fan, Yarou Hu, Yi Chen, Ruyin Tian, Cui Wang, Honghui He, Yuhang Yang, Guoming Zhang

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引用次数: 0

Abstract

Aim: To identify the potential tear fluid biomarkers in premature infants with and without retinopathy of prematurity (ROP) based on proteomic and transcriptomic analysis.

Methods: Tears were collected from the 46 eyes of the 23 enrolled premature infants, with and without ROP. Data-independent acquisition (DIA) mass spectrometry was utilized for the quantitative proteomic analysis of the two groups. Two published transcriptome datasets involving mouse oxygen-induced retinopathy (OIR) model data were selected from the Gene Expression Omnibus (GEO) database. iDEP (integrated Differential Expression and Pathway analysis) were used for differential expression analysis. Gene Ontology (GO)-based functional and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed.

Results: In this study, a total of 1742 proteins were quantified from the two groups. 55 differentially expressed proteins closely related to immune and angiogenesis processes were identified, including 33 highly expressed as well as 22 lowly expressed in the ROP group. Combined with RNA-seq data from OIR model, we screened two particularly critical proteins, LYN and filamin A (FLNA), which were both expressed at significantly elevated levels.

Conclusions: According to the findings of the tear proteomics data, we hypothesized two particularly critical proteins, LYN and FLNA, may serve as pivotal regulators of immune and angiogenesis processes in ROP. These results will assist in the provision of new potential targets for the diagnosis of ROP.

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基于蛋白质组和转录组分析鉴定早产儿视网膜病变的潜在泪液生物标志物。

目的：通过蛋白质组学和转录组学分析，确定有和无早产儿视网膜病变（ROP）的早产儿泪液中潜在的生物标志物。方法：对23例有ROP和无ROP早产儿的46只眼进行泪液采集。采用数据独立采集（DIA）质谱法对两组进行定量蛋白质组学分析。从Gene Expression Omnibus （GEO）数据库中选择两个已发表的转录组数据集，涉及小鼠氧诱导视网膜病变（OIR）模型数据。差异表达分析采用idp （integrated Differential Expression and Pathway analysis）。基于基因本体（GO）的功能分析和京都基因与基因组百科全书（KEGG）途径富集分析。结果：本研究共从两组中定量得到1742个蛋白。鉴定出55个与免疫和血管生成过程密切相关的差异表达蛋白，其中ROP组高表达33个，低表达22个。结合来自OIR模型的RNA-seq数据，我们筛选了两个特别关键的蛋白，LYN和filamin A (FLNA)，它们的表达水平都显著升高。结论：根据泪液蛋白质组学数据的发现，我们假设两个特别关键的蛋白，LYN和FLNA，可能在ROP的免疫和血管生成过程中起关键调节作用。这些结果将有助于为ROP的诊断提供新的潜在靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Graefe’s Archive for Clinical and Experimental Ophthalmology 医学-眼科学

CiteScore

5.40

自引率

7.40%

发文量

398

审稿时长

3 months

期刊介绍： Graefe''s Archive for Clinical and Experimental Ophthalmology is a distinguished international journal that presents original clinical reports and clini-cally relevant experimental studies. Founded in 1854 by Albrecht von Graefe to serve as a source of useful clinical information and a stimulus for discussion, the journal has published articles by leading ophthalmologists and vision research scientists for more than a century. With peer review by an international Editorial Board and prompt English-language publication, Graefe''s Archive provides rapid dissemination of clinical and clinically related experimental information.