Effect of DNA Aptamer Concentration on the Stability of PDA Nanoparticle-based Electrochemical Biosensor to Detect Glycated Albumin.

IF 1.8 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

In vivo Pub Date : 2025-05-01 DOI:10.21873/invivo.13945

Ohnmar Zaw, Nang Noon Shean Aye, Paweena Tunakhun, Jureerut Daduang, Sakda Daduang, Pornsuda Maraming

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引用次数: 0

Abstract

Background/aim: The glycated albumin (GA), a potential biomarker for monitoring diabetes mellitus, reflects short-term glycemia and is not influenced by conditions that falsely alter hemoglobin A1c (HbA1c) levels. This study presents a comprehensive evaluation of DNA aptamer-functionalized polydopamine nanoparticles (PDA NPs) for developing a stable biosensor targeting GA.

Materials and methods: DNA aptamers, conjugated to PDA NPs at varying aptamer concentrations (0.05, 0.5, and 5 μM), were systematically analyzed to understand their impact on the morphological, electrochemical behavior, and stable responses of the biosensor.

Results: Morphological assessments using transmission electron microscopy, scanning electron microscopy, and atomic force microscopy confirmed the stability of PDA NPs after conjugation with aptamers. Electrochemical characterization demonstrated enhanced electron transfer efficiency at an optimal aptamer concentration (0.5 μM) for GA detection while stability testing over 30 days indicated sustained sensor functionality.

Conclusion: The PDA-0.5 μM aptamer conjugations balance structural integrity, and stability, emphasizing the importance of aptamer concentration optimization for practical biosensor applications.

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DNA适体浓度对PDA纳米电化学生物传感器检测糖化白蛋白稳定性的影响。

背景/目的：糖化白蛋白（GA）是监测糖尿病的潜在生物标志物，反映短期血糖，不受虚假改变血红蛋白A1c （HbA1c）水平的条件影响。本研究对DNA适体功能化聚多巴胺纳米粒子（PDA NPs）进行了综合评价，以开发一种稳定的靶向GA的生物传感器。材料和方法：系统分析了不同浓度（0.05、0.5和5 μM）的DNA适配体对PDA NPs的形态、电化学行为和稳定响应的影响。结果：通过透射电镜、扫描电镜和原子力显微镜进行形态学评价，证实了PDA NPs与适配体结合后的稳定性。电化学表征表明，在最佳适配体浓度（0.5 μM）下，GA检测的电子传递效率提高，而超过30天的稳定性测试表明传感器功能持续。结论：PDA-0.5 μM的适体偶联体平衡了结构完整性和稳定性，强调了适体浓度优化对实际生物传感器应用的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

In vivo 医学-医学：研究与实验

CiteScore

4.20

自引率

4.30%

发文量

330

审稿时长

3-8 weeks

期刊介绍： IN VIVO is an international peer-reviewed journal designed to bring together original high quality works and reviews on experimental and clinical biomedical research within the frames of physiology, pathology and disease management. The topics of IN VIVO include: 1. Experimental development and application of new diagnostic and therapeutic procedures; 2. Pharmacological and toxicological evaluation of new drugs, drug combinations and drug delivery systems; 3. Clinical trials; 4. Development and characterization of models of biomedical research; 5. Cancer diagnosis and treatment; 6. Immunotherapy and vaccines; 7. Radiotherapy, Imaging; 8. Tissue engineering, Regenerative medicine; 9. Carcinogenesis.