Molecular detection of phenol-soluble modulin-mec (PSM-mec) in Staphylococcus aureus clinical isolates from Federal Medical Center Birnin Kebbi, North-West, Nigeria.

IF 1.6 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH

GMS Hygiene and Infection Control Pub Date : 2025-03-06 eCollection Date: 2025-01-01 DOI:10.3205/dgkh000538

Isah Musa Maishanu, Adeshina O Gbonjubola, Hussaini Mujahid, Busayo O Olayinka

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Abstract

Aim: This study was carried out to isolate and detect virulence genes associated with Staphylococcus (S.) aureus clinical isolates from the Federal Medical Center Birnin Kebbi, Nigeria.

Methods: To obtain S. aureus isolates, samples were taken from urine, sputum, blood and wound sources. S. aureus was phenotypically identified using Microgen staph ID system and PSM-mec and PVL genes were detected using polymerase chain reaction (PCR).

Results: A total of 48 non-duplicate S. aureus isolates were obtained (21 from wound swabs, 7 from blood, 15 from urine, and 5 from sputum). From the 14 S. aureus isolates examined by PCR, the most abundant gene was PSM-mec (42.8%), while the PVL was the least abundant with 21.4%.

Conclusion: Because it gives highly specific and accurate results, it is essential to use the PCR technique to detect S. aureus virulence determinants as well as PSM-mec and PVL as targets for antimicrobial agents.

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尼日利亚西北部Birnin Kebbi联邦医学中心金黄色葡萄球菌临床分离株酚溶性调素-mec的分子检测

目的：本研究旨在分离和检测来自尼日利亚Birnin Kebbi联邦医学中心的金黄色葡萄球菌临床分离株的毒力基因。方法：从尿液、痰液、血液和伤口源提取金黄色葡萄球菌分离株。采用Microgen葡萄球菌ID系统对金黄色葡萄球菌进行表型鉴定，采用聚合酶链反应（PCR）检测PSM-mec和PVL基因。结果：共分离到48株非重复金黄色葡萄球菌（伤口拭子21株，血液7株，尿液15株，痰液5株）。PCR检测的14株金黄色葡萄球菌中，PSM-mec基因丰度最高（42.8%），PVL基因丰度最低（21.4%）。结论：利用PCR技术检测金黄色葡萄球菌毒力决定因子，以及PSM-mec和PVL作为抗菌药物的靶点，具有较高的特异性和准确性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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GMS Hygiene and Infection Control PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH-

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10 weeks