Extensively acquired antimicrobial-resistant bacteria restructure the individual microbial community in post-antibiotic conditions.

IF 7.8 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

npj Biofilms and Microbiomes Pub Date : 2025-05-14 DOI:10.1038/s41522-025-00705-x

Jae Woo Baek, Songwon Lim, Nayeon Park, Byeongsop Song, Nikhil Kirtipal, Jens Nielsen, Adil Mardinoglu, Saeed Shoaie, Jae-Il Kim, Jang Won Son, Ara Koh, Sunjae Lee

{"title":"Extensively acquired antimicrobial-resistant bacteria restructure the individual microbial community in post-antibiotic conditions.","authors":"Jae Woo Baek, Songwon Lim, Nayeon Park, Byeongsop Song, Nikhil Kirtipal, Jens Nielsen, Adil Mardinoglu, Saeed Shoaie, Jae-Il Kim, Jang Won Son, Ara Koh, Sunjae Lee","doi":"10.1038/s41522-025-00705-x","DOIUrl":null,"url":null,"abstract":"<p><p>In recent years, the overuse of antibiotics has led to the emergence of antimicrobial-resistant (AMR) bacteria. To evaluate the spread of AMR bacteria, the reservoir of AMR genes (resistome) has been identified in environmental samples, hospital environments, and human populations, but the functional role of AMR bacteria and their persistence within individuals has not been fully investigated. Here, we performed a strain-resolved in-depth analysis of the resistome changes by reconstructing a large number of metagenome-assembled genomes from the gut microbiome of an antibiotic-treated individual. Interestingly, we identified two bacterial populations with different resistome profiles: extensively acquired antimicrobial-resistant bacteria (EARB) and sporadically acquired antimicrobial-resistant bacteria, and found that EARB showed broader drug resistance and a significant functional role in shaping individual microbiome composition after antibiotic treatment. Our findings of AMR bacteria would provide a new avenue for controlling the spread of AMR bacteria in the human community.</p>","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"78"},"PeriodicalIF":7.8000,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12075632/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"npj Biofilms and Microbiomes","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1038/s41522-025-00705-x","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

In recent years, the overuse of antibiotics has led to the emergence of antimicrobial-resistant (AMR) bacteria. To evaluate the spread of AMR bacteria, the reservoir of AMR genes (resistome) has been identified in environmental samples, hospital environments, and human populations, but the functional role of AMR bacteria and their persistence within individuals has not been fully investigated. Here, we performed a strain-resolved in-depth analysis of the resistome changes by reconstructing a large number of metagenome-assembled genomes from the gut microbiome of an antibiotic-treated individual. Interestingly, we identified two bacterial populations with different resistome profiles: extensively acquired antimicrobial-resistant bacteria (EARB) and sporadically acquired antimicrobial-resistant bacteria, and found that EARB showed broader drug resistance and a significant functional role in shaping individual microbiome composition after antibiotic treatment. Our findings of AMR bacteria would provide a new avenue for controlling the spread of AMR bacteria in the human community.

查看原文本刊更多论文

广泛获得的抗菌素耐药细菌重组后抗生素条件下的个体微生物群落。

近年来，抗生素的过度使用导致了抗菌素耐药细菌的出现。为了评估AMR细菌的传播，已经在环境样本、医院环境和人群中确定了AMR基因（抗性组）的储存库，但AMR细菌的功能作用及其在个体中的持久性尚未得到充分研究。在这里，我们通过从抗生素治疗个体的肠道微生物组中重建大量宏基因组组装的基因组，对抗性组变化进行了菌株解析的深入分析。有趣的是，我们确定了两种具有不同抗性组谱的细菌群体：广泛获得性抗菌素耐药细菌（EARB）和零星获得性抗菌素耐药细菌，并发现EARB在抗生素治疗后表现出更广泛的耐药性，并在塑造个体微生物组组成方面发挥重要的功能作用。我们的发现将为控制AMR细菌在人类社区的传播提供新的途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

npj Biofilms and Microbiomes Immunology and Microbiology-Microbiology

CiteScore

12.10

自引率

3.30%

发文量

审稿时长

9 weeks

期刊介绍： npj Biofilms and Microbiomes is a comprehensive platform that promotes research on biofilms and microbiomes across various scientific disciplines. The journal facilitates cross-disciplinary discussions to enhance our understanding of the biology, ecology, and communal functions of biofilms, populations, and communities. It also focuses on applications in the medical, environmental, and engineering domains. The scope of the journal encompasses all aspects of the field, ranging from cell-cell communication and single cell interactions to the microbiomes of humans, animals, plants, and natural and built environments. The journal also welcomes research on the virome, phageome, mycome, and fungome. It publishes both applied science and theoretical work. As an open access and interdisciplinary journal, its primary goal is to publish significant scientific advancements in microbial biofilms and microbiomes. The journal enables discussions that span multiple disciplines and contributes to our understanding of the social behavior of microbial biofilm populations and communities, and their impact on life, human health, and the environment.