Extraction and Characterization of Iturin A as a Key Factor in the Antagonism of Bacillus amyloliquefaciens M13RW01 toward Pathogenic Fungi.

Q3 Biochemistry, Genetics and Molecular Biology
Mahsa Aghaali Marnani, Mahboobeh Madani, Pegah Shakib
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引用次数: 0

Abstract

Background: Bacillus amyloliquefaciens contains several fungal inhibitory compounds, such as peptides and lipopeptides, representing the remarkable potential for biotechnological, agricultural, and biopharmaceutical applications.

Objectives: This research aimed to extract and characterize iturin A as the key antagonism factor of Bacillus amyloliquefaciens M13RW01 toward pathogenic fungi, using HPLC and mass spectrometry [MS] analysis.

Methods: For this study, Bacillus amyloliquefaciens strain M13-RW01 isolated from Isfahan soil was used. The lipopeptide compounds of B.amyloliquefaciens were examined for antagonistic performance against Aspergillus niger PTCC 5010, Mucor hiemalis PTCC 5292, Fusarium oxysporum CBS 62087, and Penicillium chrysogenum PTCC 5037 by well diffusion and percentage of growth inhibition. The crude extract was run on Waters μBondpak C18 column in the HPLC system to separate the antibiotics. Major antibiotics were analyzed based on MS.

Results: HPLC analysis demonstrated that the lipopeptide compound is similar to iturin A. Moreover, MS analysis of these compounds and purified iturin A revealed a high similarity between them, with the same molecular ion peaks identified. Results showed that the produced lipopeptides by Bacillus amyloliquefaciens were of iturin A genum. The molecular ion peaks of the B.amyloliquefaciens M13RW01 methanolic fraction were at 1027.10, 1043.05, 1058, 1066, 1072, 1088.95. These compounds restrained fungal germination and growth. Inhibition growth percentages were 79.28, 76.13, 84.47and 59.15% for Aspergillus niger, Mucor hiemalis, Fusarium oxysporum, and Penicillium chrysogenum, respectively.

Conclusion: According to the present study, B.amyloliquefaciens M13RW01 lipopeptides are able to inhibit the growth of some fungi. B.amyloliquefaciens M13-RW01 isolated from Isfahan soil plays an essential part in antagonizing pathogenic fungi. Thus, this antifungal lipopeptide is supposed to be a biological protection agent for farm crops.

解淀粉芽孢杆菌M13RW01拮抗病原菌关键因子Iturin A的提取及特性研究
背景:解淀粉芽孢杆菌含有多种真菌抑制化合物,如多肽和脂肽,在生物技术、农业和生物制药领域具有显著的应用潜力。目的:本研究旨在提取解淀粉芽孢杆菌M13RW01对病原菌的关键拮抗因子iturin A,并采用HPLC和质谱[MS]分析对其进行鉴定。方法:采用分离自伊斯法罕土壤的解淀粉芽孢杆菌M13-RW01菌株进行实验。通过扩散率和生长抑制率检测解淀粉芽孢杆菌脂肽化合物对黑曲霉PTCC 5010、毛霉PTCC 5292、尖孢镰刀菌CBS 62087和青霉PTCC 5037的拮抗效果。粗提物采用Waters μBondpak C18高效液相色谱柱分离抗生素。结果:高效液相色谱分析表明,脂肽化合物与iturin A相似,质谱分析表明,这些化合物与纯化的iturin A具有较高的相似性,并鉴定出相同的分子离子峰。结果表明,解淀粉芽孢杆菌所产生的脂肽属iturin A属。b.s amyloliquefaciens M13RW01甲醇组分的分子离子峰分别为1027.10、1043.05、1058、1066、1072、1088.95。这些化合物抑制真菌的萌发和生长。对黑曲霉、毛霉、尖孢镰刀菌和青霉菌的抑制率分别为79.28%、76.13%、84.47%和59.15%。结论:根据本研究,b.s amyloliquefaciens M13RW01脂肽能够抑制某些真菌的生长。从伊斯法罕土壤中分离到的解淀粉酵母菌M13-RW01在拮抗病原菌中起重要作用。因此,这种抗真菌脂肽有望成为农作物的生物保护剂。
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来源期刊
Recent patents on biotechnology
Recent patents on biotechnology Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
2.90
自引率
0.00%
发文量
51
期刊介绍: Recent Patents on Biotechnology publishes review articles by experts on recent patents on biotechnology. A selection of important and recent patents on biotechnology is also included in the journal. The journal is essential reading for all researchers involved in all fields of biotechnology.
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