A new turn off fluorescent NIR probe for hypochlorous acid and its applications.

IF 1.3 4区 化学 Q3 CHEMISTRY, MULTIDISCIPLINARY
Turkish Journal of Chemistry Pub Date : 2025-03-29 eCollection Date: 2025-01-01 DOI:10.55730/1300-0527.3725
Ayten Ibrahimova, Ayşe Nur Önem, Mehmet Altun, Emin Ahmet Yeşil, Aslı Baysal, Hasan Saygin, Ahu Soyocak, Mustafa Özyürek
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引用次数: 0

Abstract

Hypochlorous acid (HOCl) is a potent nonradical oxidant involved in various physiological processes, particularly within the human immune system. In this study, we introduce a novel, rapid, and highly efficient fluorometric method for the detection of HOCl. The method utilizes a near-infrared (NIR)-based fluorescent probe, NIR-QBH, which is characterized by its high sensitivity and chemical stability. NIR-QBH, containing olefinic C=C bonds, exhibits strong NIR emission at 660 nm (λex = 618 nm). The detection mechanism relies on the oxidation of the C=C bond in the NIR-QBH structure by HOCl, resulting in the formation of non-fluorescent products. With a detection limit of 0.23 μM, the probe demonstrates a fast response time of 4 min. Glutathione (GSH), an essential biothiol, was employed as a reference HOCl scavenger, and its HOCl scavenging activity was evaluated with an IC50 value of 8.97 μM. Furthermore, the developed fluorometric assay was successfully applied for the detection of HOCl in fetal bovine serum (FBS) and aqueous solutions.

一种新型次氯酸关闭型近红外荧光探针及其应用。
次氯酸(HOCl)是一种有效的非自由基氧化剂,参与多种生理过程,特别是在人体免疫系统中。在本研究中,我们介绍了一种新的、快速、高效的HOCl荧光检测方法。该方法采用近红外(NIR)荧光探针NIR- qbh,具有高灵敏度和化学稳定性的特点。含烯烃C=C键的NIR- qbh在660 nm (λex = 618 nm)处表现出较强的近红外发射。检测机制依赖于NIR-QBH结构中的C=C键被HOCl氧化,从而形成非荧光产物。该探针检测限为0.23 μM,响应时间为4 min。以谷胱甘肽(GSH)作为HOCl清除率的参考物,其IC50值为8.97 μM。此外,所建立的荧光测定法成功地应用于胎牛血清(FBS)和水溶液中HOCl的检测。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Turkish Journal of Chemistry
Turkish Journal of Chemistry 化学-工程:化工
CiteScore
2.40
自引率
7.10%
发文量
87
审稿时长
3 months
期刊介绍: The Turkish Journal of Chemistry is a bimonthly multidisciplinary journal published by the Scientific and Technological Research Council of Turkey (TÜBİTAK). The journal is dedicated to dissemination of knowledge in all disciplines of chemistry (organic, inorganic, physical, polymeric, technical, theoretical and analytical chemistry) as well as research at the interface with other sciences especially in chemical engineering where molecular aspects are key to the findings. The journal accepts English-language original manuscripts and contribution is open to researchers of all nationalities. The journal publishes refereed original papers, reviews, letters to editor and issues devoted to special fields. All manuscripts are peer-reviewed and electronic processing ensures accurate reproduction of text and data, plus publication times as short as possible.
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