Nitrogen cycle induced by plant growth-promoting rhizobacteria drives "microbial partners" to enhance cadmium phytoremediation.

IF 13.8 1区生物学 Q1 MICROBIOLOGY

Microbiome Pub Date : 2025-05-06 DOI:10.1186/s40168-025-02113-x

Yaowei Chi, Xianzhong Ma, Shaohua Chu, Yimin You, Xunfeng Chen, Juncai Wang, Renyuan Wang, Xia Zhang, Dongwei Zhang, Ting Zhao, Dan Zhang, Pei Zhou

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引用次数: 0

Abstract

Background: Using plant growth-promoting rhizobacteria (PGPR) combined with hyperaccumulator is an ecologically viable way to remediate cadmium (Cd) pollution in agricultural soil. Despite recent advances in elucidating PGPR-enhanced phytoremediation, the response of plant-associated microbiota to PGPR remains unclear.

Results: Here, we found that the effective colonization of PGPR reshaped the rhizosphere nutrient microenvironment, especially driving the nitrogen cycle, primarily mediated by soil nitrate reductase (S-NR). Elevated S-NR activity mobilized amino acid metabolism and synthesis pathways in the rhizosphere, subsequently driving a shift in life history strategies of the rhizosphere microbiota, and enriching specific rare taxa. The reconstructed synthetic community (SynCom3) confirmed that the inclusion of two crucial collaborators (Lysobacter and Microbacterium) could efficiently foster the colonization of PGPR and aid PGPR in executing phytoremediation enhancement. Finally, the multi-omics analysis highlighted the critical roles of phenylpropanoid biosynthesis and tryptophan metabolism pathways in inducing SynCom3 reorganization and PGPR-enhanced phytoremediation.

Conclusions: Our results underscore the significance of the rhizosphere microenvironment modification by PGPR for its colonization and efficacy, and highlight the collaborative role of rare microbiota in the context of PGPR-enhanced phytoremediation. Video Abstract.

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促进植物生长的根瘤菌诱导的氮循环驱动“微生物伙伴”增强镉的植物修复。

背景：利用植物促生根瘤菌（PGPR）与超蓄积菌（hyperaccumulator）结合是修复农业土壤镉（Cd）污染的生态可行途径。尽管最近在阐明PGPR增强植物修复方面取得了进展，但植物相关微生物群对PGPR的反应仍不清楚。结果：本研究发现，PGPR的有效定植重塑了根际营养微环境，特别是驱动氮循环，主要由土壤硝酸盐还原酶（S-NR）介导。S-NR活性的升高调动了根际氨基酸代谢和合成途径，从而推动了根际微生物群生活史策略的转变，并丰富了特定的稀有分类群。重建的合成群落（SynCom3）证实，两个关键的合作者（溶菌和微细菌）的加入可以有效地促进PGPR的定植，并帮助PGPR执行植物修复增强。最后，多组学分析强调了苯丙素生物合成和色氨酸代谢途径在诱导SynCom3重组和pgpr增强植物修复中的关键作用。结论：我们的研究结果强调了PGPR对根际微环境的修饰对其定植和效果的重要性，并强调了稀有微生物群在PGPR增强植物修复中的协同作用。视频摘要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbiome MICROBIOLOGY-

CiteScore

21.90

自引率

2.60%

发文量

198

审稿时长

4 weeks

期刊介绍： Microbiome is a journal that focuses on studies of microbiomes in humans, animals, plants, and the environment. It covers both natural and manipulated microbiomes, such as those in agriculture. The journal is interested in research that uses meta-omics approaches or novel bioinformatics tools and emphasizes the community/host interaction and structure-function relationship within the microbiome. Studies that go beyond descriptive omics surveys and include experimental or theoretical approaches will be considered for publication. The journal also encourages research that establishes cause and effect relationships and supports proposed microbiome functions. However, studies of individual microbial isolates/species without exploring their impact on the host or the complex microbiome structures and functions will not be considered for publication. Microbiome is indexed in BIOSIS, Current Contents, DOAJ, Embase, MEDLINE, PubMed, PubMed Central, and Science Citations Index Expanded.