Mahak Singh, Apanai Celina, Rahul Katiyar, Sourabh Deori, Ashwani Singh, Vinay Singh, G D Singh, J S Rajoriya, H Kalita, V K Mishra
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引用次数: 0
Abstract
In temperate regions, boars experience environmental heat stress due to the subtropical climate, leading to their semen quality and fertility being severely compromised compared to indigenous breeds. Considering the above effect, the present study aimed to evaluate the effect of season on semen quality, seminal plasma antioxidant status, and in vivo fertility of crossbred boars of exotic (50%) and indigenous inheritance in a subtropical climate. A total of 14 Hampshire-Ghungroo crossbred boars were used for this investigation, which took place in both summer and winter. Sperm characteristics, namely motility, viability, abnormality, acrosomal integrity, and the hypo-osmotic swelling test (HOST) results, and mitochondrial membrane potential (MMP) were evaluated. Sperm kinematics parameters were assessed using computer-assisted semen analysis (CASA). Antioxidant biomarkers (glutathione peroxidase, GPx; catalase, CAT; and total antioxidant capacity, TAC) and lipid peroxidation (malondialdehyde, MDA) were analyzed in boars' seminal plasma. The summer season had a significant (p < 0.01) negative impact on reaction time and false mounts, whereas semen volume and sperm concentration were significantly (p < 0.01) higher in the winter season. Similarly, sperm abnormalities were significantly (p < 0.01) lower in the winter season. In the winter, sperm quality parameters, namely total motility, progressive motility, viability, acrosomal integrity, and HOST reactivity, were significantly (p < 0.01) improved. However, during the summer, sperm MMP was significantly (p < 0.01) lower in fresh samples and after 72 h of storage. Season had a significant (p < 0.05) effect on the following sperm kinematics parameters: average path velocity, straight-line velocity, curve linear velocity, amplitude of lateral head displacement, and beat cross frequency. Semen characteristics were significantly (p < 0.01) improved in winter after 72 h of cold storage compared with those in summer. The summer season had a significant effect (p < 0.01) on seminal plasma antioxidant biomarkers (TAC, MDA, CAT, and GPx). Furthermore, the farrowing rate was significantly (p < 0.05) higher in the winter season. In conclusion, our results showed that the low MMP of boar sperm and the downregulation of seminal plasma antioxidant biomarkers in summer lead to poor semen quality and poor fertility in Hampshire-Ghungroo crossbred boars in a subtropical climate. To alleviate the heat-stress-induced poor sperm fertility in boars and to optimize the fertility of boars during summer in subtropics, there is a need for scientific interventions in terms of genetics [less exotic inheritance (below 50%)], nutrition, and management.
在温带地区，由于亚热带气候，公猪经历了环境热应激，导致它们的精液质量和生育能力与本地品种相比严重受损。考虑到上述影响，本研究旨在评价亚热带气候条件下，季节对外来（50%）和本土遗传杂交公猪精液质量、精浆抗氧化状态和体内育性的影响。在夏季和冬季进行的调查中，共有14头汉普郡-胡格鲁杂交公猪被用于调查。评估精子特征，即活力、活力、异常、顶体完整性、低渗透肿胀试验（HOST）结果和线粒体膜电位（MMP）。使用计算机辅助精液分析（CASA）评估精子运动学参数。抗氧化生物标志物(谷胱甘肽过氧化物酶，GPx；过氧化氢酶,猫;测定公猪精浆中总抗氧化能力（TAC）和脂质过氧化（丙二醛，MDA）。夏季有显著的(p p p p p p p p p
期刊介绍：
Frontiers in Veterinary Science is a global, peer-reviewed, Open Access journal that bridges animal and human health, brings a comparative approach to medical and surgical challenges, and advances innovative biotechnology and therapy.
Veterinary research today is interdisciplinary, collaborative, and socially relevant, transforming how we understand and investigate animal health and disease. Fundamental research in emerging infectious diseases, predictive genomics, stem cell therapy, and translational modelling is grounded within the integrative social context of public and environmental health, wildlife conservation, novel biomarkers, societal well-being, and cutting-edge clinical practice and specialization. Frontiers in Veterinary Science brings a 21st-century approach—networked, collaborative, and Open Access—to communicate this progress and innovation to both the specialist and to the wider audience of readers in the field.
Frontiers in Veterinary Science publishes articles on outstanding discoveries across a wide spectrum of translational, foundational, and clinical research. The journal''s mission is to bring all relevant veterinary sciences together on a single platform with the goal of improving animal and human health.
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