A novel approach to assessing the bioavailability of biopeptide inhibitor of HMG CoA reductase from germinated and ungerminated Kara Kratok (Phaseolus lunatus L.).

IF 2.3 3区生物学 Q2 MULTIDISCIPLINARY SCIENCES

PeerJ Pub Date : 2025-04-23 eCollection Date: 2025-01-01 DOI:10.7717/peerj.19262

Cahyo Budiyanto, Andriati Ningrum, Agnes Murdiati, Retno Indrati

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Abstract

Background: The bioavailability of biopeptide compounds is a development challenge, mainly because of their resistance to the digestion system. This study aimed to determine the bioavailability of HMG CoA reductase biopeptide inhibitors from germinated and ungerminated Kara Kratok (Phaseolus lunatus L.).

Methods: Germinated and ungerminated brown P. lunatus were simulated for digestion enzyme in vitro (120 minutes for pepsin and pancreatin), followed by an in situ method for absorption. Perfusate samples were measured for the absorption percentage, inhibition of HMG CoA reductase, molecular weight (MW), peptide concentration, and hydrolysis degree (%DH).

Results: The results showed that germinated brown P. lunatus exhibited the highest absorption (32.42%), and the percentage of HMG CoA reductase inhibition during enzymatic digestion was at 210 minutes (87.51%), with MW < 10 kDa, peptide concentration of 2.39 mg/mL, and %DH of 48.90%. These findings suggest that germinated brown P. lunatus is a potent HMG CoA reductase inhibitor with significantly higher bioavailability than that of its ungerminated counterpart. This finding underscores its superiority in this context and open new possibilities for biopeptide research.

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一种评估发芽和未发芽的山楂（Phaseolus lunatus L.） HMG辅酶A还原酶生物肽抑制剂生物利用度的新方法。

背景：生物肽类化合物的生物利用度是一个开发挑战，主要是因为它们对消化系统的抗性。本研究旨在测定萌发和未萌发的山楂（Phaseolus lunatus L.）中HMG辅酶a还原酶生物肽抑制剂的生物利用度。方法：体外模拟萌发和未萌发的褐月牙鲆消化酶作用（胃蛋白酶和胰酶作用120分钟），然后采用原位吸收法。测定灌注液样品的吸收率、HMG CoA还原酶抑制、分子量（MW）、肽浓度和水解度（%DH）。结果：发芽后的褐月牙草吸收率最高（32.42%），酶解过程中HMG CoA还原酶抑制率为210 min(87.51%)，褐月牙草是一种有效的HMG CoA还原酶抑制剂，生物利用度显著高于未发芽的褐月牙草。这一发现强调了其在这方面的优越性，并为生物肽的研究开辟了新的可能性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

PeerJ MULTIDISCIPLINARY SCIENCES-

CiteScore

4.70

自引率

3.70%

发文量

1665

审稿时长

10 weeks

期刊介绍： PeerJ is an open access peer-reviewed scientific journal covering research in the biological and medical sciences. At PeerJ, authors take out a lifetime publication plan (for as little as $99) which allows them to publish articles in the journal for free, forever. PeerJ has 5 Nobel Prize Winners on the Board; they have won several industry and media awards; and they are widely recognized as being one of the most interesting recent developments in academic publishing.