Identification of circular RNAs as biomarkers for pupal age estimation and postmortem interval in forensically important Sarcophaga peregrina (Diptera: Sarcophagidae).

IF 2.3 3区医学 Q1 MEDICINE, LEGAL

International Journal of Legal Medicine Pub Date : 2025-09-01 Epub Date: 2025-04-14 DOI:10.1007/s00414-025-03490-6

Hai Wu, Haojie Tang, Xing Han, Fernand Jocelin Ngando, Yanjie Shang, Yadong Guo

{"title":"Identification of circular RNAs as biomarkers for pupal age estimation and postmortem interval in forensically important Sarcophaga peregrina (Diptera: Sarcophagidae).","authors":"Hai Wu, Haojie Tang, Xing Han, Fernand Jocelin Ngando, Yanjie Shang, Yadong Guo","doi":"10.1007/s00414-025-03490-6","DOIUrl":null,"url":null,"abstract":"<p><p>Accurate estimation of the pupal age in necrophagous flies is vital for determining the postmortem interval (PMI) in forensic entomology. Differential expression of genes (DEGs) exhibits temporal fluctuations across the developmental stages of these flies. This study evaluates circular RNAs (circRNAs) as novel molecular markers and develops a model for predicting pupal development time based on circRNA expression. Transcriptomic analysis of Sarcophaga peregrina pupae at various stages identified four circRNAs (circRNA_0037, circRNA_0531, circRNA_3373, circRNA_2847) showing significant expression differences. Using real-time quantitative PCR and regression analysis, we constructed a model to estimate development time, which accurately predicts intra-puparial periods. Additionally, we examined circRNA degradation patterns in pupae under lethal conditions and identified a clear degradation trend in circRNA_2847, suggesting its potential use for estimating the PMI. This study introduces new molecular markers and insights for estimating necrophagous fly pupal age.</p>","PeriodicalId":14071,"journal":{"name":"International Journal of Legal Medicine","volume":" ","pages":"2441-2455"},"PeriodicalIF":2.3000,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Legal Medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s00414-025-03490-6","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/4/14 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"MEDICINE, LEGAL","Score":null,"Total":0}

引用次数: 0

Abstract

Accurate estimation of the pupal age in necrophagous flies is vital for determining the postmortem interval (PMI) in forensic entomology. Differential expression of genes (DEGs) exhibits temporal fluctuations across the developmental stages of these flies. This study evaluates circular RNAs (circRNAs) as novel molecular markers and develops a model for predicting pupal development time based on circRNA expression. Transcriptomic analysis of Sarcophaga peregrina pupae at various stages identified four circRNAs (circRNA_0037, circRNA_0531, circRNA_3373, circRNA_2847) showing significant expression differences. Using real-time quantitative PCR and regression analysis, we constructed a model to estimate development time, which accurately predicts intra-puparial periods. Additionally, we examined circRNA degradation patterns in pupae under lethal conditions and identified a clear degradation trend in circRNA_2847, suggesting its potential use for estimating the PMI. This study introduces new molecular markers and insights for estimating necrophagous fly pupal age.

查看原文本刊更多论文

鉴定环状rna作为鉴定具有重要法医学意义的裴氏石蛉蛹龄和死后时间的生物标志物。

尸食性蝇蛹龄的准确估计是法医昆虫学中确定死后时间间隔的关键。基因的差异表达（DEGs）在这些果蝇的发育阶段表现出时间波动。本研究评估了环状rna （circRNAs）作为新的分子标记，并建立了基于环状rna表达预测蛹发育时间的模型。转录组学分析发现四个circrna （circRNA_0037, circRNA_0531, circRNA_3373, circRNA_2847）表达差异显著。利用实时定量PCR和回归分析，构建了发育时间估算模型，该模型能准确预测蛹期。此外，我们研究了致死条件下蛹中circRNA的降解模式，并发现了circRNA_2847的明显降解趋势，这表明它可能用于估计PMI。本研究介绍了新的分子标记和估计尸食性蝇蛹年龄的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

International Journal of Legal Medicine 医学-医学：法

CiteScore

5.80

自引率

9.50%

发文量

165

审稿时长

1 months

期刊介绍： The International Journal of Legal Medicine aims to improve the scientific resources used in the elucidation of crime and related forensic applications at a high level of evidential proof. The journal offers review articles tracing development in specific areas, with up-to-date analysis; original articles discussing significant recent research results; case reports describing interesting and exceptional examples; population data; letters to the editors; and technical notes, which appear in a section originally created for rapid publication of data in the dynamic field of DNA analysis.