The protective effects of garlic (Allium sativum var. Solo Garlic) extract mitigates piroxicam-induced liver damage and oxidative stress in rats.

IF 0.9 Q3 VETERINARY SCIENCES

Open Veterinary Journal Pub Date : 2025-03-01 Epub Date: 2025-03-31 DOI:10.5455/OVJ.2025.v15.i3.26

Maya Nurwartanti Yunita, Hani Plumeriastuti, Bodhi Agustono, Moch Ilham Riza Fahlefi, Aprilia Rizki Fadilla, Ragil Ayu Pangastutie, Shelly Oktania Nurvita Sari, Muhammad Fikri Syamsuri, Muhammad Alivio Putra Fajar, Reina Puspita Rahmaniar, Aliar Rahman

{"title":"The protective effects of garlic (Allium sativum var. Solo Garlic) extract mitigates piroxicam-induced liver damage and oxidative stress in rats.","authors":"Maya Nurwartanti Yunita, Hani Plumeriastuti, Bodhi Agustono, Moch Ilham Riza Fahlefi, Aprilia Rizki Fadilla, Ragil Ayu Pangastutie, Shelly Oktania Nurvita Sari, Muhammad Fikri Syamsuri, Muhammad Alivio Putra Fajar, Reina Puspita Rahmaniar, Aliar Rahman","doi":"10.5455/OVJ.2025.v15.i3.26","DOIUrl":null,"url":null,"abstract":"Background: Piroxicam, a nonsteroidal antiinflammatory drug, is commonly used to induce inflammation in experimental models of inflammatory bowel disease (IBD). Piroxicam can modulate the immune system and disrupt the mucosal barrier, leading to increased production of reactive oxygen species.Aim: This study aimed to evaluate the effects of single-clove garlic (Allium sativum var. solo garlic) extract (SGE) on liver histopathological findings, superoxide dismutase (SOD), and malondialdehyde (MDA) levels in white rats (Rattus norvegicus) Wistar strain induced with piroxicam.Methods: The study used a completely randomized design with 30 male Wistar rats aged 2 months and weighing 150-200 g. The rats were divided into five groups: negative control (C), receiving CMC-Na 0,5% only; positive control (T0), receiving 20 mg/kgBW piroxicam; and three treatment groups, receiving 20 mg/kgBW piroxicam combined with 75 mg/kgBW (T1), 100 mg/kgBW (T2), or 125 mg/kgBW (T3) of SGE. Piroxicam was administered orally to induce inflammation, followed by SGE treatment for 14 days. Liver histopathological images were measured for damage repair using the scoring method. Superoxide dismutase and malondialdehyde levels were measured using enzyme-linked immunosorbent assay methods.Results: The results showed a significant increase in SOD levels (p < 0.05) in the treatment groups compared with the T0 (20 mg/kgBW piroxicam), with the T3 (125 mg/kgBW piroxicam) showing the most significant effect. Additionally, MDA levels were significantly decreased (p < 0.05) in all treatment groups, indicating a reduction in oxidative damage. Statistical analysis revealed a dose-dependent relationship between garlic extract administration and improvement in liver histopathological findings and oxidative stress markers.Conclusion: SGE is a potent antioxidant that reduces oxidative stress in a piroxicam-induced IBD model and contributes to good health. Further studies are needed to explore the molecular mechanisms underlying these effects and to optimize the therapeutic potential of IBD.","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":"15 3","pages":"1349-1357"},"PeriodicalIF":0.9000,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12017719/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Open Veterinary Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5455/OVJ.2025.v15.i3.26","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/3/31 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Piroxicam, a nonsteroidal antiinflammatory drug, is commonly used to induce inflammation in experimental models of inflammatory bowel disease (IBD). Piroxicam can modulate the immune system and disrupt the mucosal barrier, leading to increased production of reactive oxygen species.

Aim: This study aimed to evaluate the effects of single-clove garlic (Allium sativum var. solo garlic) extract (SGE) on liver histopathological findings, superoxide dismutase (SOD), and malondialdehyde (MDA) levels in white rats (Rattus norvegicus) Wistar strain induced with piroxicam.

Methods: The study used a completely randomized design with 30 male Wistar rats aged 2 months and weighing 150-200 g. The rats were divided into five groups: negative control (C), receiving CMC-Na 0,5% only; positive control (T0), receiving 20 mg/kgBW piroxicam; and three treatment groups, receiving 20 mg/kgBW piroxicam combined with 75 mg/kgBW (T1), 100 mg/kgBW (T2), or 125 mg/kgBW (T3) of SGE. Piroxicam was administered orally to induce inflammation, followed by SGE treatment for 14 days. Liver histopathological images were measured for damage repair using the scoring method. Superoxide dismutase and malondialdehyde levels were measured using enzyme-linked immunosorbent assay methods.

Results: The results showed a significant increase in SOD levels (p < 0.05) in the treatment groups compared with the T0 (20 mg/kgBW piroxicam), with the T3 (125 mg/kgBW piroxicam) showing the most significant effect. Additionally, MDA levels were significantly decreased (p < 0.05) in all treatment groups, indicating a reduction in oxidative damage. Statistical analysis revealed a dose-dependent relationship between garlic extract administration and improvement in liver histopathological findings and oxidative stress markers.

Conclusion: SGE is a potent antioxidant that reduces oxidative stress in a piroxicam-induced IBD model and contributes to good health. Further studies are needed to explore the molecular mechanisms underlying these effects and to optimize the therapeutic potential of IBD.

查看原文本刊更多论文

大蒜（Allium sativum var. Solo garlic）提取物减轻吡罗昔康诱导的大鼠肝损伤和氧化应激的保护作用。

背景：吡罗昔康是一种非甾体抗炎药，常用于炎症性肠病（IBD）实验模型的炎症诱导。吡罗昔康可以调节免疫系统，破坏粘膜屏障，导致活性氧的产生增加。目的：研究单瓣大蒜（Allium sativum var. solo garlic）提取物（SGE）对吡罗西康诱导大鼠Wistar肝组织病理学变化、超氧化物歧化酶（SOD）和丙二醛（MDA）水平的影响。方法：采用完全随机设计，选用30只2月龄、体重150 ~ 200 g的雄性Wistar大鼠。将大鼠分为5组：阴性对照(C)，仅给予CMC-Na 0.5%；阳性对照（T0），给予吡罗昔康20 mg/kgBW；3个治疗组，分别给予吡罗昔康20 mg/kgBW联合SGE 75 mg/kgBW （T1）、100 mg/kgBW （T2）、125 mg/kgBW （T3）。口服吡罗昔康诱导炎症，SGE治疗14 d。采用评分法对肝组织病理图像进行损伤修复。采用酶联免疫吸附法测定超氧化物歧化酶和丙二醛水平。结果：与T0组（20 mg/kgBW吡罗昔康）比较，各治疗组SOD水平均显著升高（p < 0.05），其中T3组（125 mg/kgBW吡罗昔康）效果最显著。此外，各治疗组MDA水平均显著降低（p < 0.05），表明氧化损伤减轻。统计分析显示大蒜提取物与肝脏组织病理学结果和氧化应激标志物的改善之间存在剂量依赖关系。结论：SGE是一种有效的抗氧化剂，可降低吡罗昔康诱导的IBD模型的氧化应激，有助于健康。需要进一步的研究来探索这些作用的分子机制，并优化IBD的治疗潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Open Veterinary Journal VETERINARY SCIENCES-

CiteScore

1.40

自引率

0.00%

发文量

112

审稿时长

12 weeks

期刊介绍： Open Veterinary Journal is a peer-reviewed international open access online and printed journal that publishes high-quality original research articles. reviews, short communications and case reports dedicated to all aspects of veterinary sciences and its related subjects. Research areas include the following: Infectious diseases of zoonotic/food-borne importance, applied biochemistry, parasitology, endocrinology, microbiology, immunology, pathology, pharmacology, physiology, epidemiology, molecular biology, immunogenetics, surgery, ophthalmology, dermatology, oncology and animal reproduction. All papers are peer-reviewed. Moreover, with the presence of well-qualified group of international referees, the process of publication will be done meticulously and to the highest standards.