Fluorescein sodium as a marker for focused ultrasound-induced blood-brain barrier disruption: a case report in a porcine model.

IF 1.6 4区医学 Q2 SURGERY

Frontiers in Surgery Pub Date : 2025-04-01 eCollection Date: 2025-01-01 DOI:10.3389/fsurg.2025.1559195

Yuan Xu, Thomas J On, Mark C Preul

引用次数: 0

Abstract

Transcranial low-intensity focused ultrasound (FUS) enables noninvasive, targeted, and reversible blood-brain barrier (BBB) disruption, facilitating drug delivery and liquid biopsy of the brain. Using fluorescein sodium (FNa) with macroscopic widefield fluorescence and microscopic confocal laser endomicroscopy (CLE) imaging, we assessed BBB permeability after applying a frameless, electromagnetic-guided FUS system in a porcine model and confirmed with established MRI protocol and conventional histology. Both macroscopic and microscopic FNa fluorescence imaging findings correlated with contrast-enhanced MRI, providing direct evidence of BBB disruption. This approach demonstrates the utility of FNa for evaluating BBB permeability in preclinical studies.

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荧光素钠作为聚焦超声诱导的血脑屏障破坏的标记物：猪模型的病例报告。

经颅低强度聚焦超声（FUS）可以实现无创、靶向和可逆的血脑屏障（BBB）破坏，促进药物输送和脑液体活检。利用荧光素钠（FNa）和宏观宽视场荧光和显微共聚焦激光内镜（CLE）成像，我们在猪模型上应用无框架、电磁引导的FUS系统，评估血脑屏障的通透性，并通过既定的MRI方案和常规组织学进行证实。宏观和微观FNa荧光成像结果与MRI增强相关，提供了血脑屏障破坏的直接证据。这种方法证明了FNa在临床前研究中评估血脑屏障通透性的效用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Surgery Medicine-Surgery

CiteScore

1.90

自引率

11.10%

发文量

1872

审稿时长

12 weeks

期刊介绍： Evidence of surgical interventions go back to prehistoric times. Since then, the field of surgery has developed into a complex array of specialties and procedures, particularly with the advent of microsurgery, lasers and minimally invasive techniques. The advanced skills now required from surgeons has led to ever increasing specialization, though these still share important fundamental principles. Frontiers in Surgery is the umbrella journal representing the publication interests of all surgical specialties. It is divided into several “Specialty Sections” listed below. All these sections have their own Specialty Chief Editor, Editorial Board and homepage, but all articles carry the citation Frontiers in Surgery. Frontiers in Surgery calls upon medical professionals and scientists from all surgical specialties to publish their experimental and clinical studies in this journal. By assembling all surgical specialties, which nonetheless retain their independence, under the common umbrella of Frontiers in Surgery, a powerful publication venue is created. Since there is often overlap and common ground between the different surgical specialties, assembly of all surgical disciplines into a single journal will foster a collaborative dialogue amongst the surgical community. This means that publications, which are also of interest to other surgical specialties, will reach a wider audience and have greater impact. The aim of this multidisciplinary journal is to create a discussion and knowledge platform of advances and research findings in surgical practice today to continuously improve clinical management of patients and foster innovation in this field.