Prevalence and Pathology Associated with Sarcocystis spp. in Raptors in Tennessee, USA.

IF 1.2 4区农林科学 Q3 VETERINARY SCIENCES

Journal of Wildlife Diseases Pub Date : 2025-07-01 DOI:10.7589/JWD-D-24-00130

Eliza Baker, Lani Bower, Richard Gerhold, Debra Miller

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Abstract

Raptors may serve as both intermediate and definitive hosts for Sarcocystis spp. Past research has documented fatal encephalitis in raptors caused by various Sarcocystis spp., whereas other surveys have found a high prevalence of tissue cysts without evidence of disease. Little is known about the prevalence of Sarcocystis in raptors in the eastern USA. The aim of this study was to determine the occurrence of tissue cysts and histopathologic changes associated with Sarcocystis spp. infection in raptors in eastern Tennessee. Tissues of 33 raptors from Tennessee, USA, were assessed with histopathologic examination. Cysts consistent with Sarcocystis spp. were present in the heart, skeletal, or tracheal muscle of 11 (33%) raptors, without any associated inflammation. Tissues from histopathologic-positive raptors were then tested with PCR targeting of the 18S rRNA gene of Sarcocystis. Sequence analysis of PCR products revealed that six raptors had sequences most similar to Sarcocystis falcatula, and two had sequences most similar to Sarcocystis halieti. In addition, one S. falcatula-positive Bald Eagle (Haliaeetus leucocephalus) had lymphoplasmacytic meningoencephalitis and was positive via immunohistochemistry for eastern equine encephalitis virus. Our study supports findings in other geographic regions that raptors commonly serve as hosts for Sarcocystis spp. without evidence of associated disease.

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美国田纳西州猛禽中肌囊虫病的患病率和病理学。

猛禽可能是肉囊菌的中间宿主和最终宿主。过去的研究记录了由各种肉囊菌引起的猛禽致命脑炎，而其他调查发现没有疾病证据的组织囊肿的患病率很高。美国东部猛禽中肌囊虫病的流行情况尚不清楚。本研究的目的是确定组织囊肿的发生和组织病理学变化与肉囊菌感染的猛禽在田纳西州东部。对来自美国田纳西州的33只迅猛龙进行了组织病理学检查。11只（33%）猛禽的心脏、骨骼或气管肌肉中出现与肌囊虫属一致的囊肿，未见任何相关炎症。对组织病理学阳性的猛禽组织进行肉囊菌18S rRNA基因的PCR检测。PCR产物序列分析显示，6只迅猛龙的序列与falcatula肉囊菌最相似，2只迅猛龙的序列与halieti肉囊菌最相似。另外，1只镰状芽孢杆菌阳性的白头海雕（halaeetus leucocephalus）患有淋巴浆细胞性脑膜脑炎，免疫组化结果为东部马脑炎病毒阳性。我们的研究支持了其他地理区域的发现，即猛禽通常作为肉囊菌的宿主，而没有相关疾病的证据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Wildlife Diseases 农林科学-兽医学

CiteScore

2.70

自引率

0.00%

发文量

213

审稿时长

6-16 weeks

期刊介绍： The JWD publishes reports of wildlife disease investigations, research papers, brief research notes, case and epizootic reports, review articles, and book reviews. The JWD publishes the results of original research and observations dealing with all aspects of infectious, parasitic, toxic, nutritional, physiologic, developmental and neoplastic diseases, environmental contamination, and other factors impinging on the health and survival of free-living or occasionally captive populations of wild animals, including fish, amphibians, reptiles, birds, and mammals. Papers on zoonoses involving wildlife and on chemical immobilization of wild animals are also published. Manuscripts dealing with surveys and case reports may be published in the Journal provided that they contain significant new information or have significance for better understanding health and disease in wild populations. Authors are encouraged to address the wildlife management implications of their studies, where appropriate.