A longitudinal study assessing Aspergillus polymerase chain reaction (PCR) performance through participation in external quality assessment schemes from 2019 to 2022.
Alastair Ricketts, Dorothy Montgomery, Juergen Loeffler, Rosemary Barnes, Mario Cruciani, J Peter Donnelly, P Lewis White
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引用次数: 0
Abstract
Aspergillus polymerase chain reaction (PCR) now employs standardised methodology and commercial options, with clinical performance validated through meta-analyses. The long-term availability of external quality assessment schemes drives performance and provides a currently untapped source of technical data. The Fungal PCR initiative and the Quality Control for Molecular Diagnostics (QCMD) combined forces to evaluate data from the Aspergillus PCR QCMD schemes between 2019 and 2022. Analysis showed inferior detection of non-fumigatus Aspergillus species (i.e.,Aspergillus niger) compared with A. fumigatus. Longitudinal evaluation showed the use of 'in-house' PCR methods by new participants when testing plasma generated lower positivity rates compared to commercial assays.
期刊介绍：
Medical Mycology is a peer-reviewed international journal that focuses on original and innovative basic and applied studies, as well as learned reviews on all aspects of medical, veterinary and environmental mycology as related to disease. The objective is to present the highest quality scientific reports from throughout the world on divergent topics. These topics include the phylogeny of fungal pathogens, epidemiology and public health mycology themes, new approaches in the diagnosis and treatment of mycoses including clinical trials and guidelines, pharmacology and antifungal susceptibilities, changes in taxonomy, description of new or unusual fungi associated with human or animal disease, immunology of fungal infections, vaccinology for prevention of fungal infections, pathogenesis and virulence, and the molecular biology of pathogenic fungi in vitro and in vivo, including genomics, transcriptomics, metabolomics, and proteomics. Case reports are no longer accepted. In addition, studies of natural products showing inhibitory activity against pathogenic fungi are not accepted without chemical characterization and identification of the compounds responsible for the inhibitory activity.
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