{"title":"<i>De novo</i> assembly and annotation of the <i>Empoasca fabae</i> mitochondrial genome.","authors":"Joshua Molligan, Jordanne Jacques, Soham Mukhopadhyay, Edel Pérez-López","doi":"10.1080/23802359.2025.2498740","DOIUrl":null,"url":null,"abstract":"<p><p>This study presents the assembly and annotation of the full-length mitochondrial genome for the leafhopper species <i>Empoasca fabae</i> Harris, 1841. The mitogenome was obtained from a contig-level assembly with the identified mitochondrial genome being 14,873 bp in length. The base composition was A (38.8%), T (39.1%), C (11.7%), and G (10.4%). The mitogenome comprised 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs), and showed a unique, non-AT-rich D-loop region. Phylogenetic analysis confirmed the placement of <i>E. fabae</i> within the subfamily Typhlocybinae, clustering with other species in the <i>Empoasca</i> genus.</p>","PeriodicalId":18647,"journal":{"name":"Mitochondrial DNA. Part B, Resources","volume":"10 6","pages":"403-408"},"PeriodicalIF":0.5000,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12054589/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mitochondrial DNA. Part B, Resources","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/23802359.2025.2498740","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q4","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
引用次数: 0
Abstract
This study presents the assembly and annotation of the full-length mitochondrial genome for the leafhopper species Empoasca fabae Harris, 1841. The mitogenome was obtained from a contig-level assembly with the identified mitochondrial genome being 14,873 bp in length. The base composition was A (38.8%), T (39.1%), C (11.7%), and G (10.4%). The mitogenome comprised 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs), and showed a unique, non-AT-rich D-loop region. Phylogenetic analysis confirmed the placement of E. fabae within the subfamily Typhlocybinae, clustering with other species in the Empoasca genus.
期刊介绍:
This open access journal publishes high-quality and concise research articles reporting the sequence of full mitochondrial genomes, and short communications focusing on the physical, chemical, and biochemical aspects of mtDNA and proteins involved in mtDNA metabolism and interactions.