Exploring the potential of Moringa oleifera seed extract-loaded microemulsion hydrogel in the DNCB-induced atopic dermatitis model.

IF 4.6 2区医学 Q2 IMMUNOLOGY

Inflammopharmacology Pub Date : 2025-05-10 DOI:10.1007/s10787-025-01740-y

Prakrati Garg, Aaliya Ali, Sewa Singh, Kanika Thakur, Kaisar Raza, Bigul Yogeshver Bhardwaj, Saurabh Kulshrestha, Poonam Negi

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引用次数: 0

Abstract

Background: Moringa oleifera n-hexane (MO n-Hex) seed extract contains phytoconstituents such as behenic acid, quercetin, and kaempferol. These exhibit anti-inflammatory, antiallergic, and antioxidant properties and can alleviate atopic dermatitis (AD)-like symptoms.

Purpose: This study aimed to develop and evaluate MO n-Hex seed extract-loaded microemulsion (ME) hydrogel to provide an effective, safe, non-steroidal, plant-based alternative to conventional therapies in the topical management of AD.

Methods: Optimized o/w ME was developed by building pseudo-ternary phase diagrams. Ex vivo skin penetration was determined by employing CLSM analysis. Further, skin compatibility, histological analysis, and pharmacodynamics were carried out using a DNCB-induced AD model in BALB/c mice.

Results: The best o/w ME demonstrated nearly spherical globules with size < 50 nm, zeta potential - 28.83 ± 0.492, and pH value 5.433 ± 0.047. The in vivo efficacy revealed significant improvements in AD-like symptoms, healed ear skin lesions, and lowered IgE levels and inflammatory cytokiness (IL-4, IL-5, and IFN-γ). Further, histological analysis confirmed the restoration of skin structure, supporting the formulation's potential in skin barrier repair.

Conclusions: The study demonstrated that the MO n-Hex seed extract-loaded MEs were suitable for topical use with improved penetration to deeper layers of skin while showing safety and better skin compliance. The formulated MEs effectively modulated immune responses and restored skin structure and barrier functioning.

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探讨辣木籽提取物微乳水凝胶在dncb诱导的特应性皮炎模型中的应用潜力。

背景：辣木（Moringa oleifera）正己烷（MO n-Hex）种子提取物含有甜菜酸、槲皮素和山奈酚等植物成分。它们具有抗炎、抗过敏和抗氧化的特性，可以缓解特应性皮炎（AD）样症状。目的：本研究旨在开发和评估MO n-Hex种子提取物负载微乳液（ME）水凝胶，为局部治疗AD提供一种有效、安全、非甾体、基于植物的替代疗法。方法：建立拟三元相图，优选出最佳的0 /w ME。体外皮肤穿透度采用CLSM分析。此外，采用dncb诱导的BALB/c小鼠AD模型进行皮肤相容性、组织学分析和药效学分析。结论：本研究表明，MO - n-Hex种子提取物负载的MEs适合局部使用，具有更好的渗透到更深的皮肤层，同时具有安全性和更好的皮肤依从性。配方MEs有效调节免疫反应，恢复皮肤结构和屏障功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Inflammopharmacology IMMUNOLOGYTOXICOLOGY-TOXICOLOGY

CiteScore

8.00

自引率

3.40%

发文量

200

期刊介绍： Inflammopharmacology is the official publication of the Gastrointestinal Section of the International Union of Basic and Clinical Pharmacology (IUPHAR) and the Hungarian Experimental and Clinical Pharmacology Society (HECPS). Inflammopharmacology publishes papers on all aspects of inflammation and its pharmacological control emphasizing comparisons of (a) different inflammatory states, and (b) the actions, therapeutic efficacy and safety of drugs employed in the treatment of inflammatory conditions. The comparative aspects of the types of inflammatory conditions include gastrointestinal disease (e.g. ulcerative colitis, Crohn''s disease), parasitic diseases, toxicological manifestations of the effects of drugs and environmental agents, arthritic conditions, and inflammatory effects of injury or aging on skeletal muscle. The journal has seven main interest areas: -Drug-Disease Interactions - Conditional Pharmacology - i.e. where the condition (disease or stress state) influences the therapeutic response and side (adverse) effects from anti-inflammatory drugs. Mechanisms of drug-disease and drug disease interactions and the role of different stress states -Rheumatology - particular emphasis on methods of measurement of clinical response effects of new agents, adverse effects from anti-rheumatic drugs -Gastroenterology - with particular emphasis on animal and human models, mechanisms of mucosal inflammation and ulceration and effects of novel and established anti-ulcer, anti-inflammatory agents, or antiparasitic agents -Neuro-Inflammation and Pain - model systems, pharmacology of new analgesic agents and mechanisms of neuro-inflammation and pain -Novel drugs, natural products and nutraceuticals - and their effects on inflammatory processes, especially where there are indications of novel modes action compared with conventional drugs e.g. NSAIDs -Muscle-immune interactions during inflammation [...]