Nano Milling Application of Mutamba (West Indian Elm) Leaves Extract to Enhance its In vitro Bioactivity.

Q2 Pharmacology, Toxicology and Pharmaceutics

Pharmaceutical nanotechnology Pub Date : 2025-04-29 DOI:10.2174/0122117385338502250417020845

Yuda Prasetya Nugraha, Juniar Kalpika Resmi, Muhammad Luthfi Shidik, Rachmat Mauludin, Muhamad Insanu, Sophi Damayanti, Benny Permana, Siti Farah Rahmawati, Neng Fisheri Kurniati, Nizar Happyana, Ade Danova, Heni Rachmawati

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引用次数: 0

Abstract

Background: Guazuma ulmifolia or mutamba has been traditionally used for many years as a slimming agent. Various studies reported the antihyperlipidemic activity of mutamba leaves extract due to its flavonoid content.

Objective: This research was conducted to improve the bioactivity of mutamba leaves extract by applying ball-milling technology.

Methods: Unground dried mutamba leaves were extracted in ethanol 40%. The resulting extract (ME) was nano-milled and characterized for its physicochemical parameters. The ball milling process was optimized by performing in various durations, ball and powder ratios, and rotation speed.

Results: The optimized process of ball milling produced nano-extract (NanoME) with a particle size of 492,57±55,96 nm, confirmed with particle size and SEM. Compared with ME, the crystallinity and thermal behavior of NanoME did not change by particle size reduction. The reduction of particle size also did not improve the HMG-CoA reductase inhibitor activity. ME and NanoME showed comparable activity compared to Pravastatin. However, the bioactivities of NanoME, including DPPH antioxidant activities, improved 8-fold compared to ME.

Conclusion: The improvement of these activities was attributed to the increase in their flavonoid content. This study emphasizes the role of particle size reduction or nano-extract preparation in increasing the biological activity of plant extracts.

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西印度榆树叶提取物纳米研磨提高其体外生物活性

背景：番石榴作为一种减肥剂已经使用了很多年。各种研究报道了由于黄酮类化合物的含量而产生的抗高脂血症活性。目的：采用球磨工艺提高木犀叶提取物的生物活性。方法：用40%乙醇提取未研磨的干木叶。对所得提取物（ME）进行了纳米研磨，并对其理化参数进行了表征。在不同的时间、球粉比和转速下对球磨工艺进行了优化。结果：优化后的球磨工艺制备的纳米提取物（NanoME）粒径分别为492、57±55、96 nm，经粒度和扫描电镜验证。与ME相比，纳米ome的结晶度和热行为不受粒径减小的影响。颗粒大小的减小也没有提高HMG-CoA还原酶抑制剂的活性。与普伐他汀相比，ME和NanoME的活性相当。然而，NanoME的生物活性，包括抗DPPH活性，比ME提高了8倍。结论：这些活性的提高与黄酮类化合物含量的增加有关。本研究强调了减小颗粒尺寸或纳米提取物制备在提高植物提取物生物活性方面的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pharmaceutical nanotechnology Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science

CiteScore

4.20

自引率

0.00%

发文量

期刊介绍： Pharmaceutical Nanotechnology publishes original manuscripts, full-length/mini reviews, thematic issues, rapid technical notes and commentaries that provide insights into the synthesis, characterisation and pharmaceutical (or diagnostic) application of materials at the nanoscale. The nanoscale is defined as a size range of below 1 µm. Scientific findings related to micro and macro systems with functionality residing within features defined at the nanoscale are also within the scope of the journal. Manuscripts detailing the synthesis, exhaustive characterisation, biological evaluation, clinical testing and/ or toxicological assessment of nanomaterials are of particular interest to the journal’s readership. Articles should be self contained, centred around a well founded hypothesis and should aim to showcase the pharmaceutical/ diagnostic implications of the nanotechnology approach. Manuscripts should aim, wherever possible, to demonstrate the in vivo impact of any nanotechnological intervention. As reducing a material to the nanoscale is capable of fundamentally altering the material’s properties, the journal’s readership is particularly interested in new characterisation techniques and the advanced properties that originate from this size reduction. Both bottom up and top down approaches to the realisation of nanomaterials lie within the scope of the journal.