Structural and functional analysis of a homotrimeric collagen peptide.

IF 4.3 3区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Frontiers in Bioengineering and Biotechnology Pub Date : 2025-04-28 eCollection Date: 2025-01-01 DOI:10.3389/fbioe.2025.1575341

Xinling Zhang, Kexin Li, Nan Lu, Takafumi Takebayashi, Boyu Zhou, Hongbin Xie, Yufan Li, Xingyun Long, Xingjiong Qin, Hongyi Zhao, Jiying Dong

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引用次数: 0

Abstract

Objective: This study aimed to chemically synthesize a homotrimeric collagen peptide, evaluate its safety, and assess its effectiveness in promoting collagen synthesis.

Methods: A homotrimeric collagen peptide was synthesized and structurally characterized using circular dichroism and infrared spectroscopy. Thermal stability was analyzed by TG-DSC, and molecular weight and amino acid composition were determined. In vitro cytotoxicity testing assessed safety, while UV-induced photoaging experiments evaluated its effects on collagen and elastin synthesis. In vivo studies in BALB/c mice examined its impact on collagen content, skin structure, and angiogenesis.

Results: The synthesized collagen peptide exhibited high purity (99.1%) and an amino acid composition of glycine, proline, and hydroxyproline in a balanced ratio (15:17:13). Structural analysis confirmed a stable triple-helical conformation similar to type I collagen with excellent thermal stability (Tm = 326.15°C). Cytotoxicity testing showed no adverse effects on cell viability. In vitro, the peptide significantly enhanced collagen and elastin synthesis in fibroblasts. In vivo, intradermal and subcutaneous injection increased collagen content, improved skin structure, and enhanced microvessel density.

Conclusion: This study presents a chemically synthesized homotrimeric collagen peptide with superior purity, structural stability, and biological efficacy in promoting collagen synthesis. Compared to previous studies, this biomimetic material exhibits exceptional thermal stability (Tm = 326.15°C) and a well-balanced amino acid composition, enabling applications in cosmetics and medical devices requiring heat sterilization (e.g., autoclaving), as validated by our patented method (China Patent No. ZL202410309842.9).

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同源三聚体胶原肽的结构和功能分析。

目的：本研究旨在化学合成一种同型三聚体胶原肽，评价其安全性，并评价其促进胶原合成的有效性。方法：合成一种同型三聚体胶原肽，并利用圆二色性和红外光谱对其进行结构表征。热稳定性通过TG-DSC分析，分子量和氨基酸组成测定。体外细胞毒性测试评估其安全性，而紫外线诱导光老化实验评估其对胶原蛋白和弹性蛋白合成的影响。BALB/c小鼠体内研究检测了其对胶原含量、皮肤结构和血管生成的影响。结果：合成的胶原肽纯度高（99.1%），氨基酸组成由甘氨酸、脯氨酸和羟脯氨酸组成，氨基酸比例均衡（15:17:13）。结构分析证实为稳定的三螺旋构象，类似于I型胶原，具有优异的热稳定性（Tm = 326.15°C）。细胞毒性试验显示对细胞活力无不良影响。在体外，该肽可显著促进成纤维细胞中胶原蛋白和弹性蛋白的合成。在体内，皮内和皮下注射增加胶原含量，改善皮肤结构，增强微血管密度。结论：化学合成的同型三聚体胶原肽纯度高，结构稳定，具有促进胶原合成的生物功效。与之前的研究相比，这种仿生材料具有优异的热稳定性（Tm = 326.15°C）和平衡良好的氨基酸组成，可以应用于需要热灭菌（例如高压灭菌）的化妆品和医疗设备，并通过我们的专利方法（中国专利号：201520590905）进行验证。ZL202410309842.9)。

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来源期刊

Frontiers in Bioengineering and Biotechnology Chemical Engineering-Bioengineering

CiteScore

8.30

自引率

5.30%

发文量

2270

审稿时长

12 weeks

期刊介绍： The translation of new discoveries in medicine to clinical routine has never been easy. During the second half of the last century, thanks to the progress in chemistry, biochemistry and pharmacology, we have seen the development and the application of a large number of drugs and devices aimed at the treatment of symptoms, blocking unwanted pathways and, in the case of infectious diseases, fighting the micro-organisms responsible. However, we are facing, today, a dramatic change in the therapeutic approach to pathologies and diseases. Indeed, the challenge of the present and the next decade is to fully restore the physiological status of the diseased organism and to completely regenerate tissue and organs when they are so seriously affected that treatments cannot be limited to the repression of symptoms or to the repair of damage. This is being made possible thanks to the major developments made in basic cell and molecular biology, including stem cell science, growth factor delivery, gene isolation and transfection, the advances in bioengineering and nanotechnology, including development of new biomaterials, biofabrication technologies and use of bioreactors, and the big improvements in diagnostic tools and imaging of cells, tissues and organs. In today`s world, an enhancement of communication between multidisciplinary experts, together with the promotion of joint projects and close collaborations among scientists, engineers, industry people, regulatory agencies and physicians are absolute requirements for the success of any attempt to develop and clinically apply a new biological therapy or an innovative device involving the collective use of biomaterials, cells and/or bioactive molecules. “Frontiers in Bioengineering and Biotechnology” aspires to be a forum for all people involved in the process by bridging the gap too often existing between a discovery in the basic sciences and its clinical application.