A Dual-localized Fructose Bisphosphate Aldolase is Essential for Chloroplast Development and Carbon Metabolism in Rice.

IF 4.8 1区 农林科学 Q1 AGRONOMY
Rice Pub Date : 2025-04-17 DOI:10.1186/s12284-025-00779-3
Xin Liu, Yingbo Gao, Siyuan Tang, Linli Ben, Xiaoxiang Zhang, Guichun Dong, Juan Zhou, Lingshang Lin, Zefeng Yang, Yong Zhou, Jianye Huang, Youli Yao
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Abstract

Fructose-1,6-bisphosphate aldolase (FBA) stands as a pivotal enzyme involved within the Calvin cycle and glycolytic pathways in bacteria and higher plants, but the specific function of OsFBA in rice is still unclear. Here, we identified a chloroplast and mitochondria dual-localized FBA protein, OsFBA1, in rice. Experimental evidence showed that the functionally deficient osfba1 mutants featured a notable decline in chlorophyll content, photosynthetic rate, and severe growth impediment by the three-leaf stage, leading to eventual plant demise. Up-regulation of photosynthetic-pathway genes in the osfba1 mutants indicated the essential role of OsFBA1 in chloroplast development and suggested a compensatory mechanism of other genes in the process. Furthermore, the absence of OsFBA1 impaired the carbon assimilation in young rice seedlings, and supplying exogenous glucose could partially sustain the survival of osfba1 mutant for a few more days. Pathway-specific metabolomics analysis revealed a systemic change of metabolites in the glycolytic pathway, and consequential carbohydrates accumulation due to OsFBA1 disruption. Transcriptomics profiling corroborated the expression changes of photosynthesis, and carbon metabolism pathway genes. We further demonstrated that OsFBA1 serves as the primary FBA enzyme governing energy generation, photosynthesis and carbon metabolism. These results prove that OsFBA1 is an essential core gene in supporting the life cycle of rice, its expression has to be tightly regulated.

双定位果糖二磷酸醛缩酶对水稻叶绿体发育和碳代谢至关重要。
果糖-1,6-二磷酸醛缩酶(fructose -1,6-bisphosphate aldolase, FBA)在细菌和高等植物中是参与卡尔文循环和糖酵解途径的关键酶,但在水稻中的具体功能尚不清楚。在这里,我们鉴定了水稻叶绿体和线粒体双定位的FBA蛋白OsFBA1。实验证据表明,功能缺陷的osfba1突变体在三叶期叶绿素含量和光合速率显著下降,生长障碍严重,最终导致植株死亡。osfba1突变体中光合途径基因的上调表明osfba1在叶绿体发育中起重要作用,并提示该过程中存在其他基因的补偿机制。此外,OsFBA1的缺失破坏了水稻幼苗的碳同化,外源葡萄糖的供应可以部分维持OsFBA1突变体多存活几天。途径特异性代谢组学分析揭示了糖酵解途径中代谢物的系统性变化,以及OsFBA1破坏导致的碳水化合物积累。转录组学分析证实了光合作用和碳代谢途径基因的表达变化。我们进一步证明OsFBA1是主要的FBA酶,控制能量产生、光合作用和碳代谢。这些结果证明OsFBA1是支持水稻生命周期的重要核心基因,其表达必须受到严格调控。
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来源期刊
Rice
Rice AGRONOMY-
CiteScore
10.10
自引率
3.60%
发文量
60
审稿时长
>12 weeks
期刊介绍: Rice aims to fill a glaring void in basic and applied plant science journal publishing. This journal is the world''s only high-quality serial publication for reporting current advances in rice genetics, structural and functional genomics, comparative genomics, molecular biology and physiology, molecular breeding and comparative biology. Rice welcomes review articles and original papers in all of the aforementioned areas and serves as the primary source of newly published information for researchers and students in rice and related research.
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