Electromagnetic field stimulation for long-term mobility assessment after sciatic nerve injury.

IF 1.7 4区医学 Q3 CLINICAL NEUROLOGY

Neurological Research Pub Date : 2025-05-12 DOI:10.1080/01616412.2025.2504715

J R Carrillo-Márquez, M F Carrillo-Márquez, A Ceniceros-Obregón, E Gómez-Apo, A Escobar-España, L M Rodríguez-Serrano, J D Carrillo-Ruiz

{"title":"Electromagnetic field stimulation for long-term mobility assessment after sciatic nerve injury.","authors":"J R Carrillo-Márquez, M F Carrillo-Márquez, A Ceniceros-Obregón, E Gómez-Apo, A Escobar-España, L M Rodríguez-Serrano, J D Carrillo-Ruiz","doi":"10.1080/01616412.2025.2504715","DOIUrl":null,"url":null,"abstract":"Introduction: Gauss (G) or miliTesla (mT) units measure electromagnetic field (EMF) stimulation. Tarlov Scale (TS) assesses motor impairment, Finger Abduction Scale (FAS) measures finger separation. The objective is to evaluate mobility using EMF in a Sciatic Nerve Injury (SNI) model.Materials & methods: SNI caused paresis in the right hind limb to observe motor changes in male Sprague-Dawley rats (372-529 g) (N = 30). Three groups of (n = 10) rats were created. Control Group (CG) was just injured. The experimental groups were EMF-treated two hours a day for four weeks (2/24, 5/7, 4/4), applying Gauss intensities: Low-Intensity (LIMF) 60-100 (6-10 mT) & High-Intensity (HIMF) 140-200 (14-20 mT). The mobility was measured each week with TS & FAS. Sciatic nerve & skeletal muscle histological slides were made with traditional staining technique to evaluate cellular inflammation. We used two-tailed repeated ANOVA measures & Bonferroni post hoc tests, with a significance p < 0.05 (α = 0.05) and β = 80%.Results: EMFs effectively treat SNI in rats, improving the mobility of the right hind limb in experimental groups. In the last week, TS scores were CG 3.5 (±1.35) p = 0.006, LIMF 4 (±0) p = 0.0001 & HIMF 4.3 (±0.82) p = 0.02. FAS scores were CG 0.2 (±0.42) p = 0.000001, LIMF 1.5 (±0.70) p = 0.05 & HIMF 1.8 (±0.42) p = 0.1. In histological findings, CG had inflammation in nerve & muscle, LIMF & HIMF diminished cellular activity.Discussion: TS & FAS helped to improve mobility in experimental groups using EMFs after SNI. The nerve repairing mechanism should be studied in future models.","PeriodicalId":19131,"journal":{"name":"Neurological Research","volume":" ","pages":"1-10"},"PeriodicalIF":1.7000,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Neurological Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/01616412.2025.2504715","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CLINICAL NEUROLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Introduction: Gauss (G) or miliTesla (mT) units measure electromagnetic field (EMF) stimulation. Tarlov Scale (TS) assesses motor impairment, Finger Abduction Scale (FAS) measures finger separation. The objective is to evaluate mobility using EMF in a Sciatic Nerve Injury (SNI) model.

Materials & methods: SNI caused paresis in the right hind limb to observe motor changes in male Sprague-Dawley rats (372-529 g) (N = 30). Three groups of (n = 10) rats were created. Control Group (CG) was just injured. The experimental groups were EMF-treated two hours a day for four weeks (2/24, 5/7, 4/4), applying Gauss intensities: Low-Intensity (LIMF) 60-100 (6-10 mT) & High-Intensity (HIMF) 140-200 (14-20 mT). The mobility was measured each week with TS & FAS. Sciatic nerve & skeletal muscle histological slides were made with traditional staining technique to evaluate cellular inflammation. We used two-tailed repeated ANOVA measures & Bonferroni post hoc tests, with a significance p < 0.05 (α = 0.05) and β = 80%.

Results: EMFs effectively treat SNI in rats, improving the mobility of the right hind limb in experimental groups. In the last week, TS scores were CG 3.5 (±1.35) p = 0.006, LIMF 4 (±0) p = 0.0001 & HIMF 4.3 (±0.82) p = 0.02. FAS scores were CG 0.2 (±0.42) p = 0.000001, LIMF 1.5 (±0.70) p = 0.05 & HIMF 1.8 (±0.42) p = 0.1. In histological findings, CG had inflammation in nerve & muscle, LIMF & HIMF diminished cellular activity.

Discussion: TS & FAS helped to improve mobility in experimental groups using EMFs after SNI. The nerve repairing mechanism should be studied in future models.

查看原文本刊更多论文

电磁场刺激对坐骨神经损伤后长期活动能力的评价。

简介：高斯(G)或米利特斯拉（mT）单位测量电磁场（EMF）刺激。塔洛夫量表（TS）评估运动障碍，手指外展量表（FAS）衡量手指分离。目的是利用EMF评估坐骨神经损伤（SNI）模型的移动性。材料与方法：SNI致右后肢麻痹，观察雄性Sprague-Dawley大鼠（372-529 g）运动变化（N = 30）。造三组大鼠（n = 10）。对照组（CG）为刚受伤组。实验组每天2小时，连续4周（2/24、5/7、4/4），应用高斯强度：低强度（LIMF） 60-100 （6-10 mT）和高强度（HIMF） 140-200 （14-20 mT）。每周用TS和FAS测量运动能力。采用传统染色技术制作坐骨神经和骨骼肌组织切片，评价细胞炎症反应。我们采用双尾重复方差分析和Bonferroni事后检验，结果具有显著性p。结果：电磁场有效治疗大鼠SNI，实验组右后肢活动能力得到改善。最后一周TS评分为CG 3.5（±1.35）p = 0.006, LIMF 4（±0）p = 0.0001, HIMF 4.3（±0.82）p = 0.02。FAS分数CG 0.2(±0.42)p = 0.000001, LIMF 1.5(±0.70)p = 0.05 & HIMF 1.8(±0.42)p = 0.1。在组织学上，CG有神经和肌肉炎症，LIMF和HIMF细胞活性降低。讨论：TS和FAS有助于实验组在SNI后使用emf改善活动能力。神经修复机制有待进一步研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Neurological Research 医学-临床神经学

CiteScore

3.60

自引率

0.00%

发文量

116

审稿时长

5.3 months

期刊介绍： Neurological Research is an international, peer-reviewed journal for reporting both basic and clinical research in the fields of neurosurgery, neurology, neuroengineering and neurosciences. It provides a medium for those who recognize the wider implications of their work and who wish to be informed of the relevant experience of others in related and more distant fields. The scope of the journal includes: •Stem cell applications •Molecular neuroscience •Neuropharmacology •Neuroradiology •Neurochemistry •Biomathematical models •Endovascular neurosurgery •Innovation in neurosurgery.