Deficiency of synaptotagmin-1 aggravates pressure overload-induced cardiac hypertrophy and dysfunction via the p38 MAPK signaling pathway in mice.

IF 3.4 3区 生物学 Q3 CELL BIOLOGY
Jing Shen, Junqiu Miao, Lifei Wu, Deping Wang, Guang Li, Haixiong Wang, Jimin Cao
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Abstract

Cardiac hypertrophy is a major risk factor for heart failure and sudden cardiac death, but its molecular mechanisms have not been well clarified. Synaptotagmin-1 (SYT1) is an important regulator of exocytosis and apoptosis and has been found expressed in the myocardium, while its functions in heart diseases have rarely been studied. Here, we investigated the role and mechanism of SYT1 in pressure overload-induced cardiac hypertrophy. Transverse aortic constriction (TAC) surgeries were performed to induce cardiac hypertrophy in global Syt1 knockout (Syt1+/-) mice and C57BL/6J wild-type (WT) littermates in vivo, with respective sham mice as negative controls. Cardiomyocyte hypertrophy was induced by angiotensin II (Ang II) in H9C2 cells in vitro. The results showed that SYT1 expression was significantly upregulated in WT-TAC mice and in Ang II-treated H9C2 cells. Blocking angiotensin receptor by losartan decreased SYT1 expression in Ang II-treated H9C2 cells. Syt1+/- mice showed significantly exacerbated cardiac hypertrophy, dysfunction, fibrosis, apoptosis and phosphorylation of myocardial p38 MAPK in response to TAC compared to WT mice. Knocking down SYT1 using siRNA in H9C2 cells aggravated Ang II-induced cell hypertrophy and apoptosis, and also enhanced p38 MAPK phosphorylation. Inhibition of p38 MAPK by SB203580 significantly alleviated the hypertrophy and apoptosis in Ang II-treated H9C2 cells. We conclude that deficiency of SYT1 aggravates pressure overload-induced cardiac hypertrophy via the p38 MAPK signaling pathway. The study elucidates a novel role of SYT1 in cardiac remodeling.

synaptotagmin-1缺乏可通过p38 MAPK信号通路加重小鼠压力过载诱导的心脏肥厚和功能障碍。
心脏肥厚是心衰和心源性猝死的主要危险因素,但其分子机制尚不清楚。Synaptotagmin-1 (SYT1)是胞吐和细胞凋亡的重要调节因子,已发现在心肌中表达,但其在心脏病中的功能研究甚少。在此,我们研究了SYT1在压力超负荷引起的心肌肥厚中的作用和机制。在体内对Syt1基因敲除(Syt1+/-)小鼠和C57BL/6J野生型(WT)小鼠进行主动脉横缩(TAC)手术诱导心肌肥厚,并分别以假手术小鼠为阴性对照。血管紧张素II (Ang II)在体外诱导H9C2细胞心肌肥大。结果显示,SYT1在WT-TAC小鼠和Ang ii处理的H9C2细胞中表达显著上调。氯沙坦阻断血管紧张素受体可降低Ang ii处理的H9C2细胞中SYT1的表达。与WT小鼠相比,Syt1+/-小鼠对TAC的反应显著加重了心肌肥厚、功能障碍、纤维化、细胞凋亡和心肌p38 MAPK的磷酸化。在H9C2细胞中使用siRNA敲除SYT1会加重Ang ii诱导的细胞肥大和凋亡,并增强p38 MAPK磷酸化。SB203580抑制p38 MAPK可显著减轻Ang ii处理的H9C2细胞的肥大和凋亡。我们得出结论,SYT1的缺乏通过p38 MAPK信号通路加重了压力超负荷引起的心脏肥厚。该研究阐明了SYT1在心脏重构中的新作用。
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来源期刊
Human Cell
Human Cell CELL BIOLOGY-
CiteScore
5.90
自引率
2.30%
发文量
176
审稿时长
4.5 months
期刊介绍: Human Cell is the official English-language journal of the Japan Human Cell Society. The journal serves as a forum for international research on all aspects of the human cell, encompassing not only cell biology but also pathology, cytology, and oncology, including clinical oncology. Embryonic stem cells derived from animals, regenerative medicine using animal cells, and experimental animal models with implications for human diseases are covered as well. Submissions in any of the following categories will be considered: Research Articles, Cell Lines, Rapid Communications, Reviews, and Letters to the Editor. A brief clinical case report focusing on cellular responses to pathological insults in human studies may also be submitted as a Letter to the Editor in a concise and short format. Not only basic scientists but also gynecologists, oncologists, and other clinical scientists are welcome to submit work expressing new ideas or research using human cells.
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