Assessment of the Scolicidal Effect of Bile: An Ex Vivo Study.

IF 0.9 4区医学 Q4 PARASITOLOGY

Iranian Journal of Parasitology Pub Date : 2025-01-01 DOI:10.18502/ijpa.v20i1.18108

Yahya Alperen Bayraktar, Mehmet Ali Eryılmaz, Mehmet Eşref Ulutaş, Alpaslan Şahin, Gürcan Şimşek, Şerife Yüksekkaya

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引用次数: 0

Abstract

Background: Approximately 25% of hepatic hydatid cysts rupture into the biliary tract. The precise effect of bile within the cyst on protoscoleces remains unclear. We aimed to elucidate the effect of bile on protoscoleces.

Methods: The contents of hydatid cysts from the livers of three sheep were aspirated under sterile conditions. The aspirated contents were divided into 50 separate Eppendorf tubes (5 cc). Samples from each tube were stained with 0.1% eosin Y. Pink stained protoscoleces were considered dead under light microscopy (×100). A total of 100 protoscoleces were counted in each sample, and the number of live and dead protoscoleces was recorded. The tubes were randomly divided into five groups. Group 1 served as the control, Group 2 received normal saline (NS), Group 3, received hypertonic saline, Group 4 received bile, and Group 4 received diluted bile. The number of live and dead protoscoleces was recorded at the end of the first and second hours.

Results: Compared to the initial count of live protoscoleces, the number of live protoscoleces increased at hours 1 and 2 in Groups 2 and 4. No live protoscoleces remained at hours 1 and 2 in Group 3. There was no significant change in Group 5. When compared to the control group, a significant increase in viability was observed only in Group 4 (P=0.001).

Conclusion: Bile of sheep does not exhibit scolicidal effects; rather, it positively affects protoscoleces by increasing viability.

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评估胆汁的脊柱侧弯作用：一项离体研究。

背景：大约25%的肝包虫囊肿破裂进入胆道。囊肿内胆汁对原头节的确切影响尚不清楚。我们的目的是阐明胆汁对原头节的影响。方法：在无菌条件下，从3只绵羊的肝脏中抽取包虫病内容物。抽吸内容物分为50个独立的埃彭多夫管（5cc）。每个试管的样本用0.1%的伊红y染色，光镜下粉红色染色的原头节被认为是死亡的（×100）。每个标本共计数100个原头节，并记录活的和死的原头节数。这些试管被随机分为五组。1组作为对照组，2组给予生理盐水，3组给予高渗生理盐水，4组给予胆汁，4组给予稀释后的胆汁。在第1小时和第2小时结束时记录存活和死亡的原头节数。结果：第2组和第4组的原头节存活数在第1小时和第2小时较初始计数有所增加。第3组在第1和第2小时没有存活的原头节。第5组无明显变化。与对照组相比，只有第4组的存活率显著增加（P=0.001）。结论：羊胆汁不具有脊柱侧弯作用；相反，它通过增加生存能力对原头节产生积极影响。

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来源期刊

Iranian Journal of Parasitology PARASITOLOGY-

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Iranian Journal of Parasitology (IJP) is the official publication of Iranian Society of Parasitology (ISP) launched in 2006. The society was inaugurated in 1994 and pursues the improvement of the knowledge on the parasites and parasitic diseases, exchange of scientific knowledge with foreign societies, publicity activities, and consultation on the parasitic diseases, and intimate relationship among society members. The main aims of the Journal are: contribution to the field of Parasitology, including all aspects of parasites and parasitic diseases (medical and veterinary) and related fields such as Entomology which may be submitted by scientists from Iran and all over the world.