Genetic and Antigenic Variability in VP4 and VP7 of Group A Human Rotavirus in Yunnan, China from 2015-2020.

IF 3.2 4区 医学 Q3 VIROLOGY
Intervirology Pub Date : 2025-04-29 DOI:10.1159/000546065
Haoyu Ma, Meifen Wang, Liufang Chuan, Shuying Long, Lijuan Qiu, Canchun Zhao, Qiangming Sun, Zhen Zhang, Hongchao Jiang
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引用次数: 0

Abstract

Background: Rotavirus(RVs) A is one of major reasons which causes severe dehydration diarrhea. It is also one of the high morbidity disease in children. There are only a few reports about the changes in prevalence and VP4 / VP7 genotype of RVs in southwest China.Here is the report about the prevalence of RV from 2015 to 2020 in Yunnan, southwest China.

Methods: The virus genes were extracted from RV positive samples, then VP4/VP7 genes were amplified, followed by sequencing and Gene typing, Phylogenetic analysis, antigenic epitope variation analysis and selective pressure analysis were also performed.

Results: 135 VP4 gene sequences and 143 VP7 gene sequences were obtained from stool samples during 2015 to 2020. Of them, P[8] genotype accounted for 97.0% of total, while the P[4] genotype accounted for 3.0%. As for the VP7 genotype, G9 genotype accounted for 86.0% of total, the G3 genotype accounted for 9.1%, and the G2 genotype accounted for 4.9%. G9P[8] was identified as the predominant RV strain during the epidemic season in Yunnan during 2015 to 2020. Phylogenetic analysis showed that G9 genotype sequences were primarily similar to African strains (KJ753473, KY661937), while P[8] genotype sequences were close to Southeast Asian strains (JQ837878, KX362594). In antigenic epitope variation analysis, among 37 epitope of P[8] genotype, the RotaTeq™ vaccine strain covers 31 amino acid positions, Rotarix™ covers 28 amino acid positions, while LLR covers only 9. In the representative sequence of the G9 genotype, RotaTeq™ vaccine strains cover 27 out of 29 amino acid positions, Rotarix™ cover 16 positions, and LLR cover 16 positions. The results of the selective pressure analysis indicated potential positive sites for the G9P[8] genotype located at vp7-44, vp7-100, vp7-221, vp7-278, vp4-3 and vp4-4.

Conclusions: Our study shows that G9P [8] is the most dominant rotavirus genotype in Yunnan China. Consistent with the recent epidemic trend of RV strains in China, this study could provide new perspectives on vaccine research.

云南省A组轮状病毒VP4和VP7基因遗传及抗原变异分析
背景:轮状病毒(RVs) A是引起严重脱水腹泻的主要原因之一。它也是儿童高发疾病之一。目前关于西南地区rv患病率及VP4 / VP7基因型变化的报道较少。这是2015 - 2020年云南省RV流行情况报告。方法:从RV阳性标本中提取病毒基因,扩增VP4/VP7基因,进行测序、分型、系统发育分析、抗原表位变异分析和选择压力分析。结果:2015 - 2020年共获得粪便样本VP4基因序列135条,VP7基因序列143条。其中P[8]基因型占总数的97.0%,P[4]基因型占总数的3.0%。VP7基因型中,G9基因型占86.0%,G3基因型占9.1%,G2基因型占4.9%。2015 - 2020年云南省RV流行季的优势毒株为g9p[8]。系统发育分析显示,G9基因型序列与非洲菌株(KJ753473、KY661937)较为接近,P[8]基因型序列与东南亚菌株(JQ837878、KX362594)较为接近。在抗原表位变异分析中,在P[8]基因型的37个表位中,RotaTeq™疫苗株覆盖31个氨基酸位置,Rotarix™覆盖28个氨基酸位置,LLR仅覆盖9个氨基酸位置。在G9基因型的代表性序列中,RotaTeq™疫苗株覆盖了29个氨基酸位置中的27个,Rotarix™覆盖了16个位置,LLR覆盖了16个位置。选择压力分析结果表明,G9P[8]基因型的潜在阳性位点位于vp7-44、vp7-100、vp7-221、vp7-278、vp4-3和vp4-4。结论:g9p[8]是云南省最主要的轮状病毒基因型。本研究与近年来RV毒株在中国的流行趋势一致,为疫苗研究提供了新的思路。
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来源期刊
Intervirology
Intervirology 医学-病毒学
CiteScore
5.40
自引率
0.00%
发文量
13
审稿时长
6-12 weeks
期刊介绍: ''Intervirology'' covers progress in both basic and clinical virus research, and aims to provide a forum for the various disciplines within virology. Issues publishing original papers alternate with thematic issues, focusing on clearly defined topics. This thematic concentration serves to make timely reviews, research reports and controversy easily accessible to both specialists in the field and those who want to keep track of the latest developments outside their own area of interest. In addition to original papers, regular issues publish short communications and letters to the editor to provide readers with a forum for the exchange of ideas and comments. The scope encompasses work on the molecular biology of human and animal viruses, including genome organization and regulation, and the structure and function of viral proteins. The pathogenesis, immunology, diagnosis, epidemiology, prophylaxis and therapy of viral diseases are considered.
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