High Prophylactic Efficacy of Thymol Loaded Chitosan Nanoparticles for Controlling Acute Toxoplasmosis in Mice.

IF 0.9 4区 医学 Q4 PARASITOLOGY
Mohammad Amin Ghobadi, Hossein Mahmoudvand, Amal Khudair Khalaf, Fatemeh Azadbakht, Marzieh Rashidipour, Iraj Salimikia
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引用次数: 0

Abstract

Background: Given the significant role of chitosan nanoparticles in medicine, the present study aimed to assess the in vivo efficacy of synthesized chitosan nanoparticles coated with thymol (CNCT) in combating Toxoplasma gondii infection.

Methods: Mice were administered CNCT orally at dosages ranging from 0.25 to 0.75 mg/kg/day for a duration of 14 days. Following this treatment, they were infected with T. gondii tachyzoites of the Rh strain to induce acute toxoplasmosis. Then, the mortality rate, parasite load, antioxidant activity, and the gene expression level of proinflammatory cytokines were evaluated.

Results: The dimensions of CNTN exhibit variability, with a mean size of 295 nm. The prophylactic administration of CNTN in mice infected with T. gondii resulted in a significant enhancement in survival rates and a considerable decrease in parasite load (P<0.001). The CNTN caused a significant decrease in malondialdehyde level, while a notable increase (P < 0.001) in the activity of the antioxidant enzymes. The feeding the mice infected with CNTN caused a meaningful elevation in the expression level of TNFα- and IL-1β (P<0.001). The biochemical analyses indicated no significant changes in the serum levels of liver and kidney function markers.

Conclusion: The recent study revealed that CNTN demonstrates promising in vivo effects against toxoplasmosis in murine models. These effects are attributed to its antioxidant properties and immunomodulatory capabilities, which increase specific pro-inflammatory cytokines without any noticeable signs of toxicity to liver and kidney function.

Abstract Image

Abstract Image

Abstract Image

载百里酚壳聚糖纳米颗粒防治小鼠急性弓形虫病的高预防效果。
背景:鉴于壳聚糖纳米颗粒在医学上的重要作用,本研究旨在评估合成的百里酚包被壳聚糖纳米颗粒(CNCT)在体内对抗刚地弓形虫感染的效果。方法:小鼠口服CNCT,剂量范围为0.25 ~ 0.75 mg/kg/天,持续14天。在此治疗之后,他们被Rh株的弓形虫速殖子感染,诱发急性弓形虫病。然后,评估死亡率、寄生虫负荷、抗氧化活性和促炎细胞因子的基因表达水平。结果:CNTN的尺寸呈现多变性,平均尺寸为295 nm。对感染弓形虫的小鼠预防性给予CNTN可显著提高其存活率,并显著降低其抗氧化酶活性(PP < 0.001)。经CNTN感染的小鼠喂养后,TNFα-和IL-1β的表达水平显著升高(p)。结论:最近的研究表明,CNTN在小鼠模型中具有良好的体内抗弓形虫作用。这些作用归因于其抗氧化特性和免疫调节能力,可以增加特定的促炎细胞因子,而不会对肝肾功能产生任何明显的毒性迹象。
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来源期刊
CiteScore
1.80
自引率
0.00%
发文量
55
审稿时长
6-12 weeks
期刊介绍: Iranian Journal of Parasitology (IJP) is the official publication of Iranian Society of Parasitology (ISP) launched in 2006. The society was inaugurated in 1994 and pursues the improvement of the knowledge on the parasites and parasitic diseases, exchange of scientific knowledge with foreign societies, publicity activities, and consultation on the parasitic diseases, and intimate relationship among society members. The main aims of the Journal are: contribution to the field of Parasitology, including all aspects of parasites and parasitic diseases (medical and veterinary) and related fields such as Entomology which may be submitted by scientists from Iran and all over the world.
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