Antioxidant activity and selective cytotoxicity in HCT-116 and WI-38 cell lines of LC-MS/MS profiled extract from Capparis spinosa L.

IF 3.8 3区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Frontiers in Chemistry Pub Date : 2025-04-10 eCollection Date: 2025-01-01 DOI:10.3389/fchem.2025.1540174

Amjad Ibrahim Oraibi, Ashour H Dawood, Ghada Trabelsi, Ousman B Mahamat, Leila Chekir-Ghedira, Soumaya Kilani-Jaziri

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引用次数: 0

Abstract

Introduction: Capparis spinosa L. is significant among the family Capparidaceae for its survival and tolerance to dry environments. In this study, we evaluate the antioxidant and anticancer activities of extracts of roots and aerial parts of Capparis spinosa L.

Methods: Bioactive compounds, including phenolic acids and flavonoids, in various ethyl acetate fractions from the extracted roots and aerial parts, were identified using LC-MS/MS. Principal leaf constituents characterized included Rutin, Resveratrol, Astragalin, and others. The Rutin, Resveratrol, Astragalin, (of ethyl acetate fraction), leaves, and roots were screened for antioxidant activity using DPPH, FRAP, ABTS, and CUPRAC activity assays, as well as for cytotoxicity with the MTT assay.

Result: The antioxidant and anticancer activities of the samples were evaluated using DPPH, ABTS, FRAP, CUPRAC assays, and the MTT assay. Roots and Rutin consistently exhibited the strongest antioxidant activity across all assays, with Roots (IC₅₀ = 0.06-0.36 mg/mL) excelling in FRAP and CUPRAC, and Rutin (IC₅₀ = 0.013 mg/mL) showing the highest DPPH activity. In contrast, Astragalin displayed the weakest antioxidant potential. For anticancer activity, the MTT assay revealed that Leaves (IC₅₀ = 23.26 μg/mL) and Roots (IC₅₀ = 34.65 μg/mL) were the most potent against HCT-116 cells, outperforming Nutlin (IC₅₀ = 62.72 μg/mL), with minimal toxicity to normal WI-38 cells. These results highlight the therapeutic potential of Roots and Rutin as strong antioxidant and anticancer agents.

Conclusion: The results provide useful information concerning the medicinal potentials of Capparis spinosa L., particularly about HCT-116 and WI-38 cell line selectivity, and its relevance in the synthesis of natural antioxidants.

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LC-MS/MS -MS -MS -MS -MS提取液对HCT-116和WI-38细胞株的抗氧化活性和选择性细胞毒性。

摘要/ abstract摘要：在辣椒科植物中，辣椒的生存能力和对干燥环境的耐受性具有重要意义。方法：采用液相色谱-质谱联用技术（hplc -MS/MS）对刺毛椒根和地上部位不同乙酸乙酯部位的酚酸和黄酮类化合物进行鉴定。主要成分包括芦丁、白藜芦醇、黄芪苷等。用DPPH、FRAP、ABTS和CUPRAC活性测定法筛选芦丁、白藜芦醇、黄芪苷（乙酸乙酯部分）、叶和根的抗氧化活性，并用MTT法筛选细胞毒性。结果：采用DPPH、ABTS、FRAP、CUPRAC和MTT法评价样品的抗氧化和抗癌活性。根和芦丁均表现出最强的抗氧化活性，其中根（IC50 = 0.06 ~ 0.36 mg/mL）对FRAP和CUPRAC的活性最强，芦丁（IC50 = 0.013 mg/mL）对DPPH的活性最强。黄芪甲苷的抗氧化能力最弱。在抗肿瘤活性方面，MTT试验显示，叶（IC50 = 23.26 μg/mL）和根（IC50 = 34.65 μg/mL）对HCT-116细胞的抑制作用最强，优于Nutlin (IC50 = 62.72 μg/mL)，对正常WI-38细胞的毒性最小。这些结果突出了根和芦丁作为强抗氧化剂和抗癌剂的治疗潜力。结论：研究结果揭示了辣椒的药用潜力，特别是对HCT-116和WI-38细胞系的选择性及其与天然抗氧化剂合成的相关性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Chemistry Chemistry-General Chemistry

CiteScore

8.50

自引率

3.60%

发文量

1540

审稿时长

12 weeks

期刊介绍： Frontiers in Chemistry is a high visiblity and quality journal, publishing rigorously peer-reviewed research across the chemical sciences. Field Chief Editor Steve Suib at the University of Connecticut is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to academics, industry leaders and the public worldwide. Chemistry is a branch of science that is linked to all other main fields of research. The omnipresence of Chemistry is apparent in our everyday lives from the electronic devices that we all use to communicate, to foods we eat, to our health and well-being, to the different forms of energy that we use. While there are many subtopics and specialties of Chemistry, the fundamental link in all these areas is how atoms, ions, and molecules come together and come apart in what some have come to call the “dance of life”. All specialty sections of Frontiers in Chemistry are open-access with the goal of publishing outstanding research publications, review articles, commentaries, and ideas about various aspects of Chemistry. The past forms of publication often have specific subdisciplines, most commonly of analytical, inorganic, organic and physical chemistries, but these days those lines and boxes are quite blurry and the silos of those disciplines appear to be eroding. Chemistry is important to both fundamental and applied areas of research and manufacturing, and indeed the outlines of academic versus industrial research are also often artificial. Collaborative research across all specialty areas of Chemistry is highly encouraged and supported as we move forward. These are exciting times and the field of Chemistry is an important and significant contributor to our collective knowledge.