Comparison between Inhibition of CatSper and KSper Channels with NNC 55-0396 and Quinidine on Human Sperm Function.

IF 1.6 Q2 MEDICINE, GENERAL & INTERNAL

Iranian Journal of Medical Sciences Pub Date : 2025-03-01 DOI:10.30476/ijms.2024.102383.3528

Ali Asghar Zarei, Sara Keshtgar, Masoud Haghani, Negar Firouzabadi, Mohammad Hossein Nasr-Esfahani

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引用次数: 0

Abstract

Background: Calcium enters human sperm through the "Cation Channel of Sperm" (CatSper), while potassium ions exit via the sperm potassium channel (KSper). These two channels regulate intracellular calcium concentration ([Ca²⁺]_i) and membrane potential. Our study aims to investigate and compare the contributions of these channels in capacitated sperm function.

Methods:

Results: NNC and quinidine significantly decreased progressive sperm motility (P=0.001) and reduced sperm kinematics (P=0.001). NNC but not quinidine significantly decreased sperm survival (P=0.001), reduced [Ca²⁺]_i in live spermatozoa (P=0.05), and induced the acrosomal reaction (P=0.012).

Conclusion: Inhibition of KSper without effect on [Ca²⁺]_i can inhibit sperm motility and increase mortality rate. It seems that the function of KSper is as vital as CatSper in human sperm physiology.

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NNC 55-0396和奎尼丁抑制CatSper和KSper通道对人精子功能的影响

背景：钙离子通过“精子阳离子通道”（CatSper）进入人类精子，而钾离子通过精子钾通道（KSper）退出。这两个通道调节细胞内钙离子浓度（[Ca2+]i）和膜电位。我们的研究旨在调查和比较这些通道在能性精子功能中的贡献。方法：结果：NNC和奎尼丁可显著降低精子运动能力（P=0.001）和精子运动能力（P=0.001）。NNC显著降低精子存活率（P=0.001），降低活精子[Ca2+]i (P=0.05)，诱导顶体反应（P=0.012）。结论：抑制KSper而不影响[Ca2+]i可抑制精子运动，增加死亡率。在人类精子生理中，KSper的功能似乎与CatSper一样重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian Journal of Medical Sciences MEDICINE, GENERAL & INTERNAL-

CiteScore

3.20

自引率

0.00%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Medical Sciences (IJMS) is an international quarterly biomedical publication, which is sponsored by Shiraz University of Medical Sciences. The IJMS intends to provide a scientific medium of communication for researchers throughout the globe. The journal welcomes original clinical articles as well as clinically oriented basic science research experiences on prevalent diseases in the region and analysis of various regional problems.