Changes in Contrast-Enhanced Ultrasonography Results, Vascular Endothelial Cell Damage, and Oxidative Stress Indicators in Rabbit Models With Varying Degrees of Testicular Injury

IF 2.4 4区医学 Q2 ACOUSTICS

Journal of Ultrasound in Medicine Pub Date : 2025-04-17 DOI:10.1002/jum.16704

Yaxi Zhao MS, Haifei Wang MS, Gaofeng Su MD, Yanbin Zhu BS

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引用次数: 0

Abstract

Objective

It is difficult to quickly assess the degree of testicular injury in patients with blunt testicular trauma, and accurate and rapid assessment of the degree of injury is related to clinical decision-making and treatment outcomes. This study explored the sonographic characteristics of patients with different degrees of blunt testicular trauma via contrast-enhanced ultrasound (CEUS), as well as biomarkers of oxidative stress and vascular endothelial cell injury, to further evaluate the diagnostic value of these parameters in blunt testicular trauma.

Method

New Zealand male rabbits were used to establish blunt testicular trauma models of different degrees, including contusion, hematoma, rupture, and control models.

Routine ultrasound and CEUS were performed immediately, 2, 4, and 6 h after modeling, and pathological examination was used to confirm the modeling results further. The levels of von Willebrand factor (vWF), endothelin-1 (ET-1), reactive oxygen species (ROS), and nitric oxide (NO) in the serum were analyzed.

Results

According to the CEUS results, the arrival time (AT) (F = 35.159, P < .001), time to peak intensity (TTP) (F = 122.434; P < .001), and area under the curve (AUC) (F = 47.444; P < .001) of the time-intensity curves showed significant differences among the groups. There was no significant difference between Hematoma Trauma (HT) (F = 0.178; P = .91) and Peak Intensity (PI) (F = 0.172; P = .92). For biomarkers, there were significant differences in vWF (F = 40.61; P < .001), ROS (F = 106.3; P < .001), NO (F = 79.3; P < .001), and ET-1 (F = 61.94; P < .001) levels among the groups.

Conclusions

For blunt testicular trauma, the AT, TTP, and AUC indices of CEUS can more accurately distinguish the degree of testicular injury. Oxidative stress and endothelial cell damage may also be used as biomarkers for evaluating the degree of testicular injury.

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不同程度睾丸损伤兔模型超声造影结果、血管内皮细胞损伤及氧化应激指标的变化

目的：钝性睾丸外伤患者睾丸损伤程度难以快速评估，准确快速评估损伤程度关系到临床决策和治疗效果。本研究通过对比增强超声（CEUS）探讨不同程度钝性睾丸损伤患者的超声特征，以及氧化应激和血管内皮细胞损伤的生物标志物，进一步评价这些参数对钝性睾丸损伤的诊断价值。方法：采用新西兰雄性家兔建立不同程度钝性睾丸损伤模型，包括挫伤、血肿、破裂和对照模型。造模后立即、2、4、6 h行常规超声及超声造影，病理检查进一步证实造模结果。分析血清血管性血友病因子（vWF）、内皮素-1 （ET-1）、活性氧（ROS）、一氧化氮（NO）水平。结论：对于钝性睾丸损伤，CEUS的AT、TTP、AUC指标能较准确地区分睾丸损伤程度。氧化应激和内皮细胞损伤也可作为评价睾丸损伤程度的生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Ultrasound in Medicine 医学-核医学

CiteScore

5.10

自引率

4.30%

发文量

205

审稿时长

1.5 months

期刊介绍： The Journal of Ultrasound in Medicine (JUM) is dedicated to the rapid, accurate publication of original articles dealing with all aspects of medical ultrasound, particularly its direct application to patient care but also relevant basic science, advances in instrumentation, and biological effects. The journal is an official publication of the American Institute of Ultrasound in Medicine and publishes articles in a variety of categories, including Original Research papers, Review Articles, Pictorial Essays, Technical Innovations, Case Series, Letters to the Editor, and more, from an international bevy of countries in a continual effort to showcase and promote advances in the ultrasound community. Represented through these efforts are a wide variety of disciplines of ultrasound, including, but not limited to: -Basic Science- Breast Ultrasound- Contrast-Enhanced Ultrasound- Dermatology- Echocardiography- Elastography- Emergency Medicine- Fetal Echocardiography- Gastrointestinal Ultrasound- General and Abdominal Ultrasound- Genitourinary Ultrasound- Gynecologic Ultrasound- Head and Neck Ultrasound- High Frequency Clinical and Preclinical Imaging- Interventional-Intraoperative Ultrasound- Musculoskeletal Ultrasound- Neurosonology- Obstetric Ultrasound- Ophthalmologic Ultrasound- Pediatric Ultrasound- Point-of-Care Ultrasound- Public Policy- Superficial Structures- Therapeutic Ultrasound- Ultrasound Education- Ultrasound in Global Health- Urologic Ultrasound- Vascular Ultrasound