Na Zhao, Liang Huang, Jun Ren, Mengya Zhang, Ting Yi, Hongfu Li, Hao Zhang, Bo Liu, Li Gao, Hongfei Yan, Wanquan Chen, Taiguo Liu
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引用次数: 0
Abstract
Puccinia triticina (Pt) is a heteroecious fungus needing two different plants as primary and alternate hosts throughout its life cycle. Thalictrum spp. were first identified as alternate hosts of Pt in 1921, and over 100 species have been identified. However, within China, only T. petaloideum L., T. minus L., T. minus var. hypoleucum and T. baicalense have been reported as alternate hosts of Pt. During the six-year (2015-2018, 2023-2024) field surveys in Zhangbei County (41.26°N, 115.14°E), Zhangjiakou City, Hebei Province, our research team found rust disease on T. squarrosum. This persistent infection phenomenon aroused our interest in investigating the role of T. squarrosum in the sexual reproduction of pt. To clarify whether T. squarrosum can serve as an alternate host for Pt and to analyze the source of the pathogen, this study used artificial inoculation experiments and molecular identification techniques. The results of the artificial inoculation experiments showed that the basidiospores of Pt could infect T. squarrosum, and produce pycnia on the adaxial surface of the leaf. Subsequently, aecia were produced on the abaxial side of the leaf after artificial fertilization, and the mature aecia produced aeciospores. The aeciospores were then inoculated into susceptible wheat varieties and the wheat showed typical symptoms of wheat leaf rust. These results confirmed that T. squarrosum could serve as an alternate host for Pt. For molecular identification, 20 single-aecium samples of T. squarrosum were selected. Based on sequence alignment of their ITS regions and phylogenetic analysis, it was shown that rust on T. squarrosum could be caused by infection of Pt from wheat or the species complex of P. recondita. Our study provides new insights into the sexual cycle of Pt in China and provides a scientific basis for studying the evolution of Pt virulence and optimizing control methods for wheat leaf rust.
期刊介绍：
In an ever changing world, plant science is of the utmost importance for securing the future well-being of humankind. Plants provide oxygen, food, feed, fibers, and building materials. In addition, they are a diverse source of industrial and pharmaceutical chemicals. Plants are centrally important to the health of ecosystems, and their understanding is critical for learning how to manage and maintain a sustainable biosphere. Plant science is extremely interdisciplinary, reaching from agricultural science to paleobotany, and molecular physiology to ecology. It uses the latest developments in computer science, optics, molecular biology and genomics to address challenges in model systems, agricultural crops, and ecosystems. Plant science research inquires into the form, function, development, diversity, reproduction, evolution and uses of both higher and lower plants and their interactions with other organisms throughout the biosphere. Frontiers in Plant Science welcomes outstanding contributions in any field of plant science from basic to applied research, from organismal to molecular studies, from single plant analysis to studies of populations and whole ecosystems, and from molecular to biophysical to computational approaches.
Frontiers in Plant Science publishes articles on the most outstanding discoveries across a wide research spectrum of Plant Science. The mission of Frontiers in Plant Science is to bring all relevant Plant Science areas together on a single platform.
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