Thomasclavelia ramosa and alcohol-related hepatocellular carcinoma: a microbial culturomics study.

IF 4 3区医学 Q1 GASTROENTEROLOGY & HEPATOLOGY

Gut Pathogens Pub Date : 2025-05-07 DOI:10.1186/s13099-025-00703-6

Reham Magdy Wasfy, Anissa Abdoulaye, Patrick Borentain, Babacar Mbaye, Maryam Tidjani Alou, Aurelia Caputo, Claudia Andrieu, Giovanna Mottola, Anthony Levasseur, Matthieu Million, Rene Gerolami

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Abstract

Background: Gut microbiota alteration is implicated in the pathogenesis of alcoholic liver disease (ALD) and associated hepatocellular carcinoma (HCC). No study has characterized the dysbiosis associated with ALD by microbial culturomics, which certifies viability and allows pathobiont strain candidates to be characterized.

Methods: A case-control study (n = 59) was conducted on patients with ALD without HCC (ALD-NoHCC, n = 16), ALD with HCC (ALD-HCC, n = 19) and controls (n = 24) groups. 16 S rRNA amplicon sequencing and microbial culturomics were used as complementary methods for gut microbiome profiling.

Results: Compared to the control group, Thomasclavelia ramosa and Gemmiger formicilis were significantly increased in the ALD-HCC group and Mediterraneibacter gnavus was significantly increased in the ALD-NoHCC group using 16 S rRNA sequencing. By microbial culturomics, T. ramosa was detected in all ALD samples (100%), and the most enriched since cultivated in only a small proportion of controls (20%, p < 0.001).

Conclusions: T. ramosa, identified by culturomics and 16 rRNA sequencing, may be associated with ALD and ALD-HCC. These results highlight the potential role of T. ramosa in liver cancer, in line with its genotoxic properties and its tumor growth-promoting effect in gnotobiotic mice recently reported.

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酒精相关性肝细胞癌：微生物培养组学研究

背景：肠道菌群改变与酒精性肝病（ALD）和相关肝细胞癌（HCC）的发病机制有关。没有研究通过微生物培养组学表征与ALD相关的生态失调，这证明了可行性，并允许表征候选病原体菌株。方法：对未发生HCC的ALD患者（ALD- nohcc, n = 16）、ALD合并HCC （ALD-HCC, n = 19）和对照组（n = 24）进行病例对照研究（n = 59）。采用16s rRNA扩增子测序和微生物培养组学作为肠道微生物组分析的补充方法。结果：16s rRNA测序结果显示，与对照组相比，ALD-HCC组中tomasclavelia ramosa和Gemmiger formicilis显著升高，ALD-NoHCC组中gnavus地中海杆菌显著升高。通过微生物培养组学，所有ALD样品中均检测到拉莫沙菌（100%），仅在一小部分对照中（20%）培养后富集程度最高，p结论：通过培养组学和16 rRNA测序鉴定，拉莫沙菌可能与ALD和ALD- hcc有关。这些结果强调了T. ramosa在肝癌中的潜在作用，与其最近报道的基因毒性和促肿瘤生长作用一致。

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来源期刊

Gut Pathogens GASTROENTEROLOGY & HEPATOLOGY-MICROBIOLOGY

CiteScore

7.70

自引率

2.40%

发文量

期刊介绍： Gut Pathogens is a fast publishing, inclusive and prominent international journal which recognizes the need for a publishing platform uniquely tailored to reflect the full breadth of research in the biology and medicine of pathogens, commensals and functional microbiota of the gut. The journal publishes basic, clinical and cutting-edge research on all aspects of the above mentioned organisms including probiotic bacteria and yeasts and their products. The scope also covers the related ecology, molecular genetics, physiology and epidemiology of these microbes. The journal actively invites timely reports on the novel aspects of genomics, metagenomics, microbiota profiling and systems biology. Gut Pathogens will also consider, at the discretion of the editors, descriptive studies identifying a new genome sequence of a gut microbe or a series of related microbes (such as those obtained from new hosts, niches, settings, outbreaks and epidemics) and those obtained from single or multiple hosts at one or different time points (chronological evolution).