Molecular Detection and Genotyping of Blastocystis in Farmed Minks and Raccoon Dogs in Northern China.

IF 1.9 2区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY
Nian-Yu Xue, Yanchun Wang, Hai-Tao Wang, Qing-Yu Hou, Ya Qin, Jing Jiang, Yan Tang
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Abstract

Blastocystis is a common intestinal protozoan found in both humans and animals. However, research on Blastocystis infections in minks (Neovison vison) and raccoon dogs (Nyctereutes procyonoides) is limited. This study aimed to investigate the prevalence and subtypes of Blastocystis in these two species across northern provinces of China using small subunit rRNA gene sequence analysis. We examined 275 minks (89 from Hebei Province, 57 from Heilongjiang Province, 109 from Liaoning Province, and 20 from Shandong Province) and 235 raccoon dogs (114 from Hebei Province, 27 from Heilongjiang Province, 61 from Liaoning Province, and 33 from Jilin Province). The overall infection rate was 13.5% (69/510). Farmed minks had a prevalence of 1.5% (4/275), while raccoon dogs showed a significantly higher prevalence of 27.7% (65/235). We identified five distinct subtypes (ST1, ST2, ST3, ST5, and potentially novel subtype) across both species: ST1 (15 cases), ST2 (15 cases), ST3 (31 cases), ST5 (7 cases), and potentially novel subtype (1 case). This study is the first to report ST1 and ST3 in minks and ST1, ST2, ST5, and potentially novel subtype in raccoon dogs. As ST1, ST2, ST3, and ST5 subtypes are also found in humans, our findings suggest that minks and raccoon dogs may serve as potential reservoirs for human Blastocystis infections. Furthermore, the newly identified, potentially novel subtype might also be transmissible to humans or other animals. This research enhances our understanding of Blastocystis distribution among hosts in China and supports zoonotic disease prevention efforts related to farmed minks and raccoon dogs.

中国北方养殖水貂和貉囊虫的分子检测和基因分型。
囊虫是一种常见的肠道原生动物,存在于人类和动物体内。然而,对水貂(Neovison vison)和貉(Nyctereutes procyonoides)囊虫感染的研究有限。本研究旨在通过小亚基rRNA基因序列分析,了解中国北方省份这两个物种囊虫的流行情况和亚型。我们检测了275只水貂(河北89只、黑龙江57只、辽宁109只、山东20只)和235只貉(河北114只、黑龙江27只、辽宁61只、吉林33只)。总感染率为13.5%(69/510)。养殖水貂患病率为1.5%(4/275),而貉患病率为27.7%(65/235),显著高于养殖水貂。我们在两个物种中发现了五种不同的亚型(ST1、ST2、ST3、ST5和潜在的新亚型):ST1(15例)、ST2(15例)、ST3(31例)、ST5(7例)和潜在的新亚型(1例)。本研究首次报道了水貂中的ST1和ST3,以及貉中的ST1、ST2、ST5和潜在的新亚型。由于在人类中也发现了ST1、ST2、ST3和ST5亚型,我们的研究结果表明水貂和貉可能是人类囊虫感染的潜在宿主。此外,新发现的潜在新型亚型也可能传播给人类或其他动物。本研究提高了我们对囊虫在中国宿主中的分布的认识,并为与养殖水貂和貉有关的人畜共患疾病的预防工作提供了支持。
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来源期刊
Foodborne pathogens and disease
Foodborne pathogens and disease 医学-食品科技
CiteScore
5.30
自引率
3.60%
发文量
80
审稿时长
1 months
期刊介绍: Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide. Foodborne Pathogens and Disease coverage includes: Agroterrorism Safety of organically grown and genetically modified foods Emerging pathogens Emergence of drug resistance Methods and technology for rapid and accurate detection Strategies to destroy or control foodborne pathogens Novel strategies for the prevention and control of plant and animal diseases that impact food safety Biosecurity issues and the implications of new regulatory guidelines Impact of changing lifestyles and consumer demands on food safety.
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