Quantifying senescence, death rates, and lifespans of trematode parthenitae.

Abstract

For many trematode species, individual reproductive parthenitae in first intermediate host colonies senesce, die, and are replaced by newly born parthenitae. The times involved in these processes are poorly understood. Here, we present an approach to estimate parthenita death rates and lifespans that uses readily obtainable data on senescent parthenita frequencies, brood sizes, and offspring (cercaria) release rates. The onset of parthenita senescence is often marked by the degeneration and disappearance of the germinal mass, its source of new offspring. Following germinal mass loss, the remaining viable offspring in a senescent parthenita finish development and are birthed before parthenita death. Therefore, a senescing parthenita's remaining lifespan is the time it takes for all its viable offspring to mature and exit. We can estimate this time by measuring whole-colony (infected snail) cercaria shed rates, dissecting colonies to count reproductives, and then apply the per redia cercaria production rate to the observed brood sizes of senescent parthenitae. The per-capita parthenita death rate is then calculated as the proportion of parthenitae that are senescent divided by their average remaining lifespan. Reproductive parthenita lifespan is the inverse of this death rate. We demonstrate the approach using philophthalmid trematodes, first providing documentation of a free-floating germinal mass in 4 philophthalmids, and then, for 3 of those species, estimating parthenita senescence rates, death rates, and lifespans. This method should be broadly applicable among trematode species and help inform our understanding of trematode colony dynamics, social structure, and the evolution of parthenita senescence.