Exploring the infection strategy of Colletotrichum fructicola in pecan and two effectors Cf-ID1 and Cf-ID2 were characterized using unique molecular identifier-RNA sequencing technology.
Long-Jiao Hu, Ji-Ping Xuan, Yang Li, Min Zhai, Guo-Ming Wang, Li-Na Deng, Zheng-Hai Mo
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引用次数: 0
Abstract
The anthracnose disease caused by Colletotrichum fructicola has widely occurred in pecan (Carya illinoinensis) in China, seriously affecting its fruit yield and quality. However, the details of the infection strategy of C. fructicola remain to be elucidated. In this study, unique molecular identifier-RNA sequencing (UMI RNA-seq) was used to analyze differentially expressed genes (DEGs) of C. fructicola and candidate effectors were predicted. Two candidate effectors were identified during the early infection stages of C. fructicola. There were 6,822 DEGs at three infection timepoints (6, 24, and 36 h post-inoculation), and these genes were involved in spore germination, nutrient uptake, detoxification, secretion of toxic substances (such as effectors and toxins), inhibition of the host's immune response, and protein post-translational modification, which participated in the pathogenic process of C. fructicola. Moreover, 191 candidate effectors were predicted and their expression trends were divided into five clusters. Two candidate effectors Cf-ID1 and Cf-ID2 were selected for functional validation, and they were demonstrated to trigger cell death and immune response in Nicotiana benthamiana. Cf-ID1 and Cf-ID2 are located in both cytoplasm and nucleus and could suppress the infection of C. fructicola by eliciting defense responses in N. benthamiana. This study provided valuable information for in-depth research on the pathogenesis of C. fructicola.
期刊介绍:
In an ever changing world, plant science is of the utmost importance for securing the future well-being of humankind. Plants provide oxygen, food, feed, fibers, and building materials. In addition, they are a diverse source of industrial and pharmaceutical chemicals. Plants are centrally important to the health of ecosystems, and their understanding is critical for learning how to manage and maintain a sustainable biosphere. Plant science is extremely interdisciplinary, reaching from agricultural science to paleobotany, and molecular physiology to ecology. It uses the latest developments in computer science, optics, molecular biology and genomics to address challenges in model systems, agricultural crops, and ecosystems. Plant science research inquires into the form, function, development, diversity, reproduction, evolution and uses of both higher and lower plants and their interactions with other organisms throughout the biosphere. Frontiers in Plant Science welcomes outstanding contributions in any field of plant science from basic to applied research, from organismal to molecular studies, from single plant analysis to studies of populations and whole ecosystems, and from molecular to biophysical to computational approaches.
Frontiers in Plant Science publishes articles on the most outstanding discoveries across a wide research spectrum of Plant Science. The mission of Frontiers in Plant Science is to bring all relevant Plant Science areas together on a single platform.