Spontaneously Diabetic Torii Fatty Rat Shows Early Stage of Diabetic Retinopathy Characterized by Capillary Changes and Inflammation.

IF 3.4 3区医学 Q2 ENDOCRINOLOGY & METABOLISM

Journal of Diabetes Research Pub Date : 2025-04-21 eCollection Date: 2025-01-01 DOI:10.1155/jdr/3800292

Kasumi Kikuchi, Miyuki Murata, Yasushi Kageyama, Masami Shinohara, Tomohiko Sasase, Kousuke Noda, Susumu Ishida

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Abstract

Purpose: The Spontaneously Diabetic Torii (SDT) fatty rat is an animal model of obese Type 2 diabetes. We previously reported that the SDT fatty rats develop diabetic cataracts. This study aimed to elucidate early diabetic changes in the retina of the SDT fatty rats. Materials and Methods: The retinal thickness, capillary diameter, and pericyte/endothelial cell (P/E) ratio were assessed in the male SDT fatty rats and Sprague-Dawley (SD) rats at 24 weeks of age. Immunostaining was performed to assess the intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) levels in the retinal capillaries. DNA microarray analysis was performed to detect inflammation-associated molecules in the retina of the SDT fatty rats. Real-time PCR and Magnetic Luminex Assay were performed to validate the results. Results: The retinal thickness in the SDT fatty rats was significantly greater than that in SD rats. The capillary diameter in the retina of the SDT fatty rats was significantly higher than that of SD rats. The P/E ratio in the SDT fatty rats was significantly lower than that in SD rats. ICAM-1 and VCAM-1 were observed in the retinal vessels of the SDT fatty rats. The levels of mRNA and protein of Mcp1, Il1b, Icam1, and Tnf were upregulated in the retinal tissues of the 24-week-old SDT fatty rats. Conclusions: Our study demonstrated that the SDT fatty rats exhibited early diabetic retinal changes, suggesting that the SDT fatty rats may be useful in research on the pathogenesis of early human diabetic retinopathy.

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自发性糖尿病Torii脂肪大鼠早期糖尿病视网膜病变表现为毛细血管改变和炎症。

目的：自发性糖尿病Torii （SDT）肥胖大鼠是肥胖型2型糖尿病的动物模型。我们之前报道过SDT脂肪大鼠会发生糖尿病性白内障。本研究旨在阐明SDT肥胖大鼠视网膜在糖尿病早期的变化。材料与方法：测定24周龄雄性SDT脂肪大鼠和SD大鼠的视网膜厚度、毛细血管直径和周细胞/内皮细胞（P/E）比值。免疫染色检测视网膜毛细血管细胞间黏附分子-1 （ICAM-1）和血管细胞黏附分子-1 （VCAM-1）水平。采用DNA微阵列分析检测SDT肥胖大鼠视网膜炎症相关分子。采用Real-time PCR和Magnetic Luminex Assay对结果进行验证。结果：SDT脂肪大鼠视网膜厚度明显大于SD大鼠。SDT脂肪大鼠视网膜毛细血管直径明显高于SD大鼠。SDT脂肪大鼠的市盈率明显低于SD大鼠。在SDT肥胖大鼠视网膜血管中观察到ICAM-1和VCAM-1的表达。24周龄SDT脂肪大鼠视网膜组织中Mcp1、Il1b、Icam1和Tnf的mRNA和蛋白水平上调。结论：我们的研究表明，SDT脂肪大鼠出现了早期糖尿病视网膜病变，提示SDT脂肪大鼠可能有助于研究早期人类糖尿病视网膜病变的发病机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Diabetes Research ENDOCRINOLOGY & METABOLISM-MEDICINE, RESEARCH & EXPERIMENTAL

CiteScore

8.40

自引率

2.30%

发文量

152

审稿时长

14 weeks

期刊介绍： Journal of Diabetes Research is a peer-reviewed, Open Access journal that publishes research articles, review articles, and clinical studies related to type 1 and type 2 diabetes. The journal welcomes submissions focusing on the epidemiology, etiology, pathogenesis, management, and prevention of diabetes, as well as associated complications, such as diabetic retinopathy, neuropathy and nephropathy.