Emergence and characterization of mixed Candida auris strain infections in China.

IF 3.6 2区医学 Q1 INFECTIOUS DISEASES

Infection Pub Date : 2025-10-01 Epub Date: 2025-04-14 DOI:10.1007/s15010-025-02536-6

Han Du, Yanfeng Huang, Penghao Guo, Weihong Liang, Tianhong Zheng, Zhangyue Guan, Jian Bing, Haiqing Chu, Guanghua Huang

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引用次数: 0

Abstract

Purpose: The multidrug-resistant fungal pathogen Candida auris poses an increasing global health threat due to its high transmissibility and persistence in healthcare environments. We aimed to explore the potential cases of mixed C. auris-strain colonizations or infections in China and to investigate the genetic and biological diversity of the associated isolates.

Methods: C. auris isolates from 5 colonization or infection cases were distinguished by colony morphology and verified by D1/D2-ITS alignment. Phylogenetic and genomic diversity analysis of all isolates were conducted using whole genome sequences. Comparative biological analysis of all isolates, including cellular and colony morphology, antifungal susceptibility, biofilm formation, SAP activity, and both in vitro and in vivo survival capabilities were performed.

Results: Five cases of potential mixed C. auris-strain colonization or infections in China were identified. Comparative genomic analysis revealed these cases involved strains from two distinct genetic clades (I and III) or strains from the same clade but with genetic alterations. Comparative biological analysis demonstrated the strains from mixed colonization or infections exhibit differences in several key aspects, including colony morphology, biofilm formation, SAP activity, and both in vitro and in vivo survival capabilities.

Conclusion: Comparative analyses revealed notable differences in biofilm formation, environmental survival, and secretion of virulence factors between the co-colonizing or co-infecting strains of C. auris. These biological and genetic disparities may present significant challenges for the diagnosis and treatment of C. auris infections, as strains with different genetic backgrounds may exhibit varying abilities to colonize host or environmental niches.

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中国混合耳念珠菌感染的发生及特征分析。

目的：耐多药真菌病原体耳念珠菌由于其在卫生保健环境中的高传染性和持久性，对全球健康构成越来越大的威胁。我们的目的是探索中国可能存在的金黄色葡萄球菌混合菌种定植或感染病例，并调查相关分离株的遗传和生物学多样性。方法：用菌落形态和D1/D2-ITS比对方法对5例定植或感染病例分离的金黄色葡萄球菌进行鉴定。利用全基因组序列对所有分离株进行系统发育和基因组多样性分析。对所有分离株进行比较生物学分析，包括细胞和菌落形态、抗真菌敏感性、生物膜形成、SAP活性以及体外和体内生存能力。结果：在中国鉴定出5例可能的混合耳c菌定植或感染病例。比较基因组分析显示，这些病例涉及来自两个不同遗传枝（I和III）的菌株或来自同一进化枝但遗传改变的菌株。比较生物学分析表明，来自混合定植或感染的菌株在几个关键方面表现出差异，包括菌落形态、生物膜形成、SAP活性以及体外和体内生存能力。结论：通过比较分析发现，共定殖菌株和共感染菌株在生物膜形成、环境存活和毒力因子分泌方面存在显著差异。这些生物学和遗传差异可能对耳球菌感染的诊断和治疗提出重大挑战，因为具有不同遗传背景的菌株可能表现出不同的定植宿主或环境生态位的能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Infection 医学-传染病学

CiteScore

12.50

自引率

1.30%

发文量

224

审稿时长

6-12 weeks

期刊介绍： Infection is a journal dedicated to serving as a global forum for the presentation and discussion of clinically relevant information on infectious diseases. Its primary goal is to engage readers and contributors from various regions around the world in the exchange of knowledge about the etiology, pathogenesis, diagnosis, and treatment of infectious diseases, both in outpatient and inpatient settings. The journal covers a wide range of topics, including: Etiology: The study of the causes of infectious diseases. Pathogenesis: The process by which an infectious agent causes disease. Diagnosis: The methods and techniques used to identify infectious diseases. Treatment: The medical interventions and strategies employed to treat infectious diseases. Public Health: Issues of local, regional, or international significance related to infectious diseases, including prevention, control, and management strategies. Hospital Epidemiology: The study of the spread of infectious diseases within healthcare settings and the measures to prevent nosocomial infections. In addition to these, Infection also includes a specialized "Images" section, which focuses on high-quality visual content, such as images, photographs, and microscopic slides, accompanied by brief abstracts. This section is designed to highlight the clinical and diagnostic value of visual aids in the field of infectious diseases, as many conditions present with characteristic clinical signs that can be diagnosed through inspection, and imaging and microscopy are crucial for accurate diagnosis. The journal's comprehensive approach ensures that it remains a valuable resource for healthcare professionals and researchers in the field of infectious diseases.