Identification of mitophagy-related genes in patients with acute myocardial infarction.

IF 2.5 3区生物学

Hereditas Pub Date : 2025-04-26 DOI:10.1186/s41065-025-00424-5

Ju-Ying Li, Hong-Kui Chen, Yi-Hao Huang, Yu-Peng Zhi, Yue-E Li, Kai-Yang Lin, Chun Chen, Yan-Song Guo

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Abstract

Mitophagy is involved in acute myocardial infarction (AMI) process. However, the role of mitophagy-related genes (MRGs) in the AMI process is not well illustrated. We identified MRGs involved in AMI by bioinformatics analysis. The external datasets were employed for the validation of the MRGs, alongside the execution of cellular and animal experiments. Forty-five MRGs were detected, and machine learning identified the top four hub genes, namely ALDH2, ACSL1, IL1B, and GABARAPL1. Additionally, an external validation set was used to screen for three diagnostic markers (ACSL1, IL1B, and GABARAPL1) among these hub genes. Immune infiltration analysis revealed changes in the immune microenvironment among patients with AMI. Finally, the significant upregulation of ACSL1, IL1B, and GABARAPL1 in both cellular and animal models was confirmed. The occurrence of mitophagy was observed in the cell model through transmission electron microscopy (TEM). Our study demonstrated that ACSL1, IL1B, and GABARAPL1 possess potential biomarkers for AMI.

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急性心肌梗死患者线粒体自噬相关基因的鉴定。

线粒体自噬参与急性心肌梗死（AMI）过程。然而，线粒体自噬相关基因（MRGs）在AMI过程中的作用尚未得到很好的阐明。我们通过生物信息学分析确定了与AMI相关的mrg。外部数据集用于核磁共振成像的验证，以及细胞和动物实验的执行。检测到45个mrg，机器学习确定了前四个中心基因，即ALDH2， ACSL1， IL1B和GABARAPL1。此外，使用外部验证集筛选这些中心基因中的三个诊断标记（ACSL1， IL1B和GABARAPL1）。免疫浸润分析揭示急性心肌梗死患者免疫微环境的变化。最后，证实ACSL1、IL1B和GABARAPL1在细胞和动物模型中的显著上调。透射电镜观察细胞模型中有丝分裂的发生。我们的研究表明ACSL1、IL1B和GABARAPL1具有潜在的AMI生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Hereditas Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.80

自引率

3.70%

发文量

期刊介绍： For almost a century, Hereditas has published original cutting-edge research and reviews. As the Official journal of the Mendelian Society of Lund, the journal welcomes research from across all areas of genetics and genomics. Topics of interest include human and medical genetics, animal and plant genetics, microbial genetics, agriculture and bioinformatics.