Non-contact electrical stimulation via a Vector-potential transformer promotes bone healing in drill-hole injury model.

IF 2.4 3区医学 Q3 ENDOCRINOLOGY & METABOLISM

Journal of Bone and Mineral Metabolism Pub Date : 2025-04-29 DOI:10.1007/s00774-025-01603-0

Nao Yashima, Wataru Minamizono, Hiroya Matsunaga, Jiazheng Lyu, Kaoru Fujikawa, Hirai Suito, Takumi Okunuki, Shingo Nakai, Masafumi Ohsako

{"title":"Non-contact electrical stimulation via a Vector-potential transformer promotes bone healing in drill-hole injury model.","authors":"Nao Yashima, Wataru Minamizono, Hiroya Matsunaga, Jiazheng Lyu, Kaoru Fujikawa, Hirai Suito, Takumi Okunuki, Shingo Nakai, Masafumi Ohsako","doi":"10.1007/s00774-025-01603-0","DOIUrl":null,"url":null,"abstract":"Introduction: We investigated the effects of non-contact electrical stimulation via a Vector-potential (VP) transformer, a novel physical therapy device, on bone healing in drill-hole injury models.Materials and methods: Six-week-old male Wistar rats, after a one-week acclimation period, were divided into three groups: the control group (CO), the bone injury group (BI), in which a drill-hole injury was created, and the VP stimulation group (VP), which received non-contact electrical stimulation via a VP transformer after bone injury. In the VP group, rats underwent stimulation at 200 kHz for 30 minutes per day, seven days per week.Results: The VP group exhibited increased bone formation as early as day 7 post-injury, with significantly higher bone volume than the BI group at all time points (day 7: p = 0.0003; day 14: p = 0.0024; day 21: p = 0.0001). By day 21, the VP group showed lighter toluidine blue staining and reduced biglycan immunoreactivity compared to the BI group. Bone mineral density also increased (p = 0.0008). Osteoblasts in the VP group displayed abundant cytoplasm and a high capacity for osteocalcin synthesis. Additionally, the VP group demonstrated increased expression of Bglap (day 5: p = 0.0068; day 7: p = 0.0096) and Ctsk (day 7: p = 0.0329; day 14: p = 0.0171), along with a higher number of TRAP-positive osteoclasts (day 21: p = 0.0159) compared to the BI group.Conclusion: Non-contact electrical stimulation via a VP transformer promotes bone healing in drill-hole injury models.","PeriodicalId":15116,"journal":{"name":"Journal of Bone and Mineral Metabolism","volume":" ","pages":""},"PeriodicalIF":2.4000,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Bone and Mineral Metabolism","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s00774-025-01603-0","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENDOCRINOLOGY & METABOLISM","Score":null,"Total":0}

引用次数: 0

Abstract

Introduction: We investigated the effects of non-contact electrical stimulation via a Vector-potential (VP) transformer, a novel physical therapy device, on bone healing in drill-hole injury models.

Materials and methods: Six-week-old male Wistar rats, after a one-week acclimation period, were divided into three groups: the control group (CO), the bone injury group (BI), in which a drill-hole injury was created, and the VP stimulation group (VP), which received non-contact electrical stimulation via a VP transformer after bone injury. In the VP group, rats underwent stimulation at 200 kHz for 30 minutes per day, seven days per week.

Results: The VP group exhibited increased bone formation as early as day 7 post-injury, with significantly higher bone volume than the BI group at all time points (day 7: p = 0.0003; day 14: p = 0.0024; day 21: p = 0.0001). By day 21, the VP group showed lighter toluidine blue staining and reduced biglycan immunoreactivity compared to the BI group. Bone mineral density also increased (p = 0.0008). Osteoblasts in the VP group displayed abundant cytoplasm and a high capacity for osteocalcin synthesis. Additionally, the VP group demonstrated increased expression of Bglap (day 5: p = 0.0068; day 7: p = 0.0096) and Ctsk (day 7: p = 0.0329; day 14: p = 0.0171), along with a higher number of TRAP-positive osteoclasts (day 21: p = 0.0159) compared to the BI group.

Conclusion: Non-contact electrical stimulation via a VP transformer promotes bone healing in drill-hole injury models.

查看原文本刊更多论文

通过矢量电位变压器的非接触电刺激促进钻孔损伤模型骨愈合。

简介：我们研究了一种新型物理治疗装置——矢量电位（VP）变压器的非接触电刺激对钻孔损伤模型骨愈合的影响。材料与方法：6周龄雄性Wistar大鼠经过1周的驯化后，分为3组：对照组（CO）、骨损伤组（BI）和VP刺激组（VP），分别在骨损伤后通过VP变压器进行非接触电刺激。在VP组，大鼠接受200 kHz的刺激，每天30分钟，每周7天。结果：VP组早在损伤后第7天骨形成增加，各时间点骨体积均显著高于BI组(第7天：p = 0.0003；第14天：p = 0.0024；第21天：p = 0.0001)。到第21天，与BI组相比，VP组的甲苯胺蓝染色较轻，biglycan免疫反应性降低。骨密度升高（p = 0.0008）。VP组成骨细胞表现出丰富的细胞质和高的骨钙素合成能力。此外，VP组Bglap表达增加(第5天：p = 0.0068；第7天：p = 0.0096)和Ctsk(第7天：p = 0.0329；第14天：p = 0.0171)，与BI组相比，trap阳性破骨细胞数量更高（第21天：p = 0.0159）。结论：通过VP变压器非接触电刺激促进钻孔损伤模型骨愈合。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Bone and Mineral Metabolism 医学-内分泌学与代谢

CiteScore

6.30

自引率

3.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The Journal of Bone and Mineral Metabolism (JBMM) provides an international forum for researchers and clinicians to present and discuss topics relevant to bone, teeth, and mineral metabolism, as well as joint and musculoskeletal disorders. The journal welcomes the submission of manuscripts from any country. Membership in the society is not a prerequisite for submission. Acceptance is based on the originality, significance, and validity of the material presented. The journal is aimed at researchers and clinicians dedicated to improvements in research, development, and patient-care in the fields of bone and mineral metabolism.