Expression and prognostic value of estrogen-related receptor β alternative splicing isoforms in esophageal squamous cell carcinoma.

IF 2.8 3区医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL

European Journal of Medical Research Pub Date : 2025-05-07 DOI:10.1186/s40001-025-02638-9

Kai-Yuan Jiang, Jia-Xin Wan, Hong-Yun Li, Wei-Yang Chen, Chun-Mei Shen, Ke-Xuan Guo, Xiang-Yun Zheng, Hong-Ying Wen, Dong Tian

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引用次数: 0

Abstract

Background: Estrogen-related receptor β (ERRβ) alternative splicing isoforms, including ERRβsf, ERRβ2, and ERRβΔ10, have been implicated in the pathogenesis of malignant tumors. Nevertheless, their specific impact on esophageal squamous cell carcinoma (ESCC) remains unclear. The study aimed to investigate the expression and prognostic value of ERRβ alternative splicing isoforms in ESCC.

Methods: This study prospectively collected ESCC tissues and paired normal tissues of 54 patients with ESCC who underwent esophagectomy without neoadjuvant therapy. The protein expression levels of ERRβ alternative splicing isoforms in ESCC and normal tissues were detected by Western blot. The Kaplan-Meier method with a log-rank test was used to estimate overall survival (OS). The Cox proportional hazards regression analysis was used to evaluate the independent prognostic factors.

Results: In ESCC tissues, the ERRβsf/ERRβ ratio was significantly higher (P = 0.017) compared to paired normal tissues. Based on a cut-off value of 0.24, there were 18 and 36 cases in the high ERRβsf/ERRβ expression group and low ERRβsf/ERRβ expression group, respectively. Patients with high ERRβsf/ERRβ ratios had significantly better OS than those patients with low ERRβsf/ERRβ ratios (77.1% vs 50.8%, P = 0.024). The multivariate analysis revealed that ERRβsf/ERRβ (hazard ratio [HR] = 0.219, 95% confidence interval [CI] 0.063-0.767, P = 0.018) and N stage (HR = 7.892, 95% CI 1.328-46.911, P = 0.023) were independent prognostic factors for ESCC patients.

Conclusions: This study is the first to demonstrate the relationship between ERRβ alternative splicing isoforms in ESCC. A high ERRβsf/ERRβ ratio was associated with a better prognosis, indicating that ERRβ alternative splicing isoforms may serve as potential prognostic biomarkers for ESCC.

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雌激素相关受体β选择性剪接异构体在食管鳞状细胞癌中的表达及预后价值

背景：雌激素相关受体β (ERRβ)选择性剪接异构体，包括ERRβsf、ERRβ2和ERRβΔ10，已被认为与恶性肿瘤的发病机制有关。然而，它们对食管鳞状细胞癌（ESCC）的具体影响尚不清楚。本研究旨在探讨ERRβ选择性剪接异构体在ESCC中的表达及其预后价值。方法：本研究前瞻性收集54例未经新辅助治疗的食管切除术ESCC患者的ESCC组织和配对正常组织。Western blot检测erβ选择性剪接异构体在ESCC和正常组织中的表达水平。采用Kaplan-Meier法和log-rank检验估计总生存期（OS）。采用Cox比例风险回归分析评价独立预后因素。结果：ESCC组织中ERRβsf/ERRβ比值显著高于配对正常组织（P = 0.017）。截断值为0.24，ERRβsf/ERRβ高表达组18例，ERRβsf/ERRβ低表达组36例。高ERRβsf/ERRβ比值患者的OS明显优于低ERRβsf/ERRβ比值患者（77.1% vs 50.8%, P = 0.024）。多因素分析显示，ERRβsf/ERRβ（风险比[HR] = 0.219, 95%可信区间[CI] 0.063 ~ 0.767, P = 0.018）和N分期（HR = 7.892, 95% CI 1.338 ~ 46.911, P = 0.023）是ESCC患者的独立预后因素。结论：本研究首次证实了erβ选择性剪接异构体在ESCC中的关系。较高的ERRβsf/ERRβ比值与较好的预后相关，表明ERRβ选择性剪接异构体可能作为ESCC的潜在预后生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

European Journal of Medical Research 医学-医学：研究与实验

CiteScore

3.20

自引率

0.00%

发文量

247

审稿时长

>12 weeks

期刊介绍： European Journal of Medical Research publishes translational and clinical research of international interest across all medical disciplines, enabling clinicians and other researchers to learn about developments and innovations within these disciplines and across the boundaries between disciplines. The journal publishes high quality research and reviews and aims to ensure that the results of all well-conducted research are published, regardless of their outcome.