Using Electroosmotic Pumps to Control the Flow Velocity in Cotton Thread-Based µTADs.

IF 3 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

ELECTROPHORESIS Pub Date : 2025-05-10 DOI:10.1002/elps.8153

Xionghui Li, Haonan Li, Xuanying Liang, Zejingqiu Chen, Muyang Zhang, Qinghao He, Jie Zhou, Zitao Feng, Yeqian Liu, Xinyi Chen, Huilin Chen, Zitong Ye, Ziwei Huang, Xingwei Zhang, Huiru Zhang, Lok Ting Chu, Weijin Guo

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引用次数: 0

Abstract

Flow velocity control is of great interest for passive microfluidic devices that are used in point-of-care diagnostics. Various methods have been developed for the flow velocity control of microfluidic paper-based analytical devices (µPADs), whereas fewer attempts have been made for microfluidic thread-based analytical devices (µTADs). In this research, we attempt to control the flow velocity in cotton thread-based µTADs with electroosmotic pumping. Utilizing electroosmotic pumps, the flow velocity in the cotton thread-based µTADs can be decreased or increased by 13% and 106%, respectively. Moreover, the dynamic control of the flow velocity in the cotton thread-based µTADs is achieved by adjusting the real-time magnitude and direction of the voltage. Furthermore, we demonstrate that electroosmotic pumps can be used to overcome the hydrophobic valves in the cotton thread-based µTADs. We show that the delivery sequence of different liquid samples for a three-branch µTAD can be controlled. Finally, we show the potential application in lithium detection with a colorimetric assay. This method for flow velocity control shows promise for customizing the flow velocity and reaction time of cotton thread-based µTADs, and this method can potentially increase the sensitivity of detection.

查看原文本刊更多论文

利用电渗透泵控制棉线微tad的流速。

流速控制是非常感兴趣的被动微流体装置，用于点护理诊断。基于微流控纸的分析装置（µPADs）的流速控制方法已经开发出来，而基于微流控线的分析装置（µTADs）的流速控制方法却很少。在这项研究中，我们尝试用电渗泵来控制棉线基µTADs的流速。使用电渗透泵，棉线基µTADs的流速可以分别降低或提高13%和106%。此外，通过实时调节电压的大小和方向，实现了基于棉线的µTADs中流速的动态控制。此外，我们证明了电渗透泵可以用来克服基于棉线的µTADs中的疏水阀。我们证明了三分支µTAD的不同液体样品的递送顺序可以控制。最后，我们展示了用比色法检测锂的潜在应用。这种流速控制方法有望定制基于棉线的µTADs的流速和反应时间，并且该方法可以潜在地提高检测灵敏度。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ELECTROPHORESIS 生物-分析化学

CiteScore

6.30

自引率

13.80%

发文量

244

审稿时长

1.9 months

期刊介绍： ELECTROPHORESIS is an international journal that publishes original manuscripts on all aspects of electrophoresis, and liquid phase separations (e.g., HPLC, micro- and nano-LC, UHPLC, micro- and nano-fluidics, liquid-phase micro-extractions, etc.). Topics include new or improved analytical and preparative methods, sample preparation, development of theory, and innovative applications of electrophoretic and liquid phase separations methods in the study of nucleic acids, proteins, carbohydrates natural products, pharmaceuticals, food analysis, environmental species and other compounds of importance to the life sciences. Papers in the areas of microfluidics and proteomics, which are not limited to electrophoresis-based methods, will also be accepted for publication. Contributions focused on hyphenated and omics techniques are also of interest. Proteomics is within the scope, if related to its fundamentals and new technical approaches. Proteomics applications are only considered in particular cases. Papers describing the application of standard electrophoretic methods will not be considered. Papers on nanoanalysis intended for publication in ELECTROPHORESIS should focus on one or more of the following topics: • Nanoscale electrokinetics and phenomena related to electric double layer and/or confinement in nano-sized geometry • Single cell and subcellular analysis • Nanosensors and ultrasensitive detection aspects (e.g., involving quantum dots, "nanoelectrodes" or nanospray MS) • Nanoscale/nanopore DNA sequencing (next generation sequencing) • Micro- and nanoscale sample preparation • Nanoparticles and cells analyses by dielectrophoresis • Separation-based analysis using nanoparticles, nanotubes and nanowires.