Protective Effect of Enterococcus faecium Against Alcohol-Induced Acute Liver Injury Via Extracellular Vesicles in Rats.

IF 1.9 2区农林科学 Q3 FOOD SCIENCE & TECHNOLOGY

Foodborne pathogens and disease Pub Date : 2025-04-21 DOI:10.1089/fpd.2025.0005

Yuanyuan Zhu, Xiaofang Zhang, WenHui Huang, Meiying Luo, Xin Feng, Huihua Zhang, Qien Qi

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Abstract

The beneficial effects of probiotics on alcohol-induced liver injury have been studied, but the mechanisms by which Enterococcus regulates liver function are still under investigation. In this study, we examined Enterococcus faecium (Efm) and E. faecium-derived extracellular vesicles (EfmEVs) to provide a protective effect against ethanol-induced liver injury in rats. We evaluated the impact of EfmEVs on liver histological lesions, antioxidative function, alanine aminotransferase (ALT), aspartate aminotransferase (AST) activities, and serum ALT, AST, blood alcohol concentration. The results demonstrated that pretreatment with Efm significantly ameliorated ethanol-induced liver injury. Efm pretreatment mitigated the decline in ethanol-induced liver antioxidant indicators (malondialdehyde, superoxide dismutase, and glutathione peroxidase. Additionally, Efm pretreatment significantly reduced ethanol-induced ALT activities in the liver and serum, potentially by lowering blood ethanol concentration. Further, functional studies on three bioactive components (inactivated Efm, EfmEVs, and EVs-free supernatants) from the bacterial culture revealed that EVs were primarily responsible for the liver-protective effect. Moreover, EVs secretion contributed to the overall liver-protective effect of Efm. In summary, EfmEVs mediated the protective effect of Efm against ethanol-induced liver injury, potentially by improving antioxidative function and lowering blood ethanol concentration. These findings suggest that EfmEVs could serve as a potential antioxidative strategy to alleviate alcohol-induced acute liver injury.

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屎肠球菌通过细胞外囊泡对大鼠酒精性急性肝损伤的保护作用。

益生菌对酒精性肝损伤的有益作用已被研究，但肠球菌调节肝功能的机制仍在研究中。在这项研究中，我们检测了屎肠球菌（Efm）和屎肠球菌衍生的细胞外囊泡（efmev）对乙醇诱导的大鼠肝损伤的保护作用。我们评估了efmev对肝脏组织病变、抗氧化功能、丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）活性、血清ALT、AST、血液酒精浓度的影响。结果表明，Efm预处理可显著改善乙醇性肝损伤。Efm预处理减轻了乙醇诱导的肝脏抗氧化指标（丙二醛、超氧化物歧化酶和谷胱甘肽过氧化物酶）的下降。此外，Efm预处理显著降低了肝脏和血清中乙醇诱导的ALT活性，可能是通过降低血液中乙醇浓度来实现的。此外，对细菌培养物中三种生物活性成分（灭活Efm、efmev和不含ev的上清液）的功能研究表明，ev主要负责肝脏保护作用。此外，EVs的分泌有助于Efm的整体肝脏保护作用。综上所述，efmev介导了Efm对乙醇性肝损伤的保护作用，可能是通过改善抗氧化功能和降低血乙醇浓度来实现的。这些发现表明，efmev可能作为一种潜在的抗氧化策略，以减轻酒精诱导的急性肝损伤。

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来源期刊

Foodborne pathogens and disease 医学-食品科技

CiteScore

5.30

自引率

3.60%

发文量

审稿时长

1 months

期刊介绍： Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide. Foodborne Pathogens and Disease coverage includes: Agroterrorism Safety of organically grown and genetically modified foods Emerging pathogens Emergence of drug resistance Methods and technology for rapid and accurate detection Strategies to destroy or control foodborne pathogens Novel strategies for the prevention and control of plant and animal diseases that impact food safety Biosecurity issues and the implications of new regulatory guidelines Impact of changing lifestyles and consumer demands on food safety.