A Methyl-Accepting Chemotaxis Protein MCP-5685 Associated with Indole Synthesis in Pantoea ananatis YJ76 Influences its Plant Growth-Promoting Potential and Adaptability to Stress Conditions.

Abstract

Pantoea ananatis YJ76 is a predominant endophytic diazotrophic bacterium isolated from rice, which can produce indole as a signal to improve stress resistance, colonization, and growth-promoting effect on the host. Methyl-accepting chemotaxis proteins (MCPs) are the main chemoreceptor in bacteria and participate in regulating various cellular activities. By constructing an mTn5 transposon mutant library of YJ76, we screened out a mutant with decreased indole production. And its ability to resist stresses and starvation, as well as colonizing and growth-promoting effect on host rice plants, was inhibited. Using the hiTAIL-PCR technique combined the genome re-sequencing, the mutation site was identified as the mcp-5685 gene with a length of 1545 bp. Bioinformatics analysis and chemotaxis experiments showed that the MCP encoded by mcp-5685 had L-serine chemotaxis functions, revealing the mechanism of the gene encoding protein to drive L-serine uptake, a key component for tryptophan synthesis, and thus promote indole synthesis in the regulatory pathways for indole synthesis. Starting from the upstream regulation direction of indole synthesis, this study breaks through the previous researching limitation of focusing only on the downstream physiological function regulation of indole and provides new ideas for studying the indole signal.