Activity of cefiderocol on extensively drug-resistant Pseudomonas aeruginosa from burn wound infections in Mansoura, Egypt.

IF 1.7 Q4 MICROBIOLOGY

Iranian Journal of Microbiology Pub Date : 2025-04-01 DOI:10.18502/ijm.v17i2.18384

Rasha El-Mahdy, Ahmed Mostafa, Nora El-Tantawy, Raghdaa Shrief

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引用次数: 0

Abstract

Background and objectives: Increased Pseudomonas aeruginosa antibiotic resistance limits treatment options and is associated with a higher level of mortality and mordacity. The purpose of this research was to identify class 1 and 2 integrons, carbapenemase, SHV, and TEM genes in extensively drug-resistant (XDR) P. aeruginosa isolated from infected burns and evaluate their in vitro cefiderocol activity.

Materials and methods: By using the disc diffusion method, the antimicrobial susceptibility of 110 P. aeruginosa isolates collected from infected burns were evaluated. XDR P. aeruginosa were screened phenotypically for carbapenemase and extended spectrum β-lactamases (ESBLs) production. Both MIC Test Strip and disc diffusion were employed to test the cefiderocol susceptibility. PCR was used to assess carbapenemase, SHV and TEM genes and integrons class 1 and 2.

Results: From the 110 P. aeruginosa, 54 isolates (49%) were XDR. TEM gene was detected in 35 isolates. Among XDR isolates, carbapenemase genes were detected in 31.5%, with NDM being predominant Thirty XDR isolates had class1 integrons. All isolates were sensitive to cefiderocol and its MIC₅₀/MIC₉₀ was 0.5/1.5mg/L (range 0.064-1.5mg/L).

Conclusion: Nearly half the P. aeruginosa isolates from burn infections were extensively drug-resistant. Cefiderocol's in vitro activity demonstrated that it is a promising therapy alternative for treating extensively drug-resistant P. aeruginosa in burn patients.

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头孢地罗对埃及曼苏拉烧伤感染广泛耐药铜绿假单胞菌的作用。

背景和目的：铜绿假单胞菌抗生素耐药性的增加限制了治疗选择，并与较高的死亡率和发病率相关。本研究的目的是鉴定广泛耐药（XDR）铜绿假单胞菌（P. aeruginosa）中1类和2类整合子、碳青霉烯酶、SHV和TEM基因，并评估它们的体外头孢醚活性。材料与方法：采用纸片扩散法对110株铜绿假单胞菌进行药敏评价。对XDR P. aeruginosa进行了碳青霉烯酶和广谱β-内酰胺酶（ESBLs）生产的表型筛选。采用MIC试纸条和纸片扩散法检测头孢地罗的药敏。PCR检测碳青霉烯酶、SHV和TEM基因及1类和2类整合子。结果：110株铜绿假单胞菌中有54株为XDR，占49%。在35株分离株中检测到TEM基因。在XDR分离株中，碳青霉烯酶基因占31.5%，以NDM基因为主。所有菌株对头孢地罗均敏感，MIC50/MIC90为0.5/1.5mg/L（范围为0.064 ~ 1.5mg/L）。结论：烧伤感染中近半数铜绿假单胞菌具有广泛耐药。头孢地罗的体外活性表明，它是治疗烧伤患者广泛耐药铜绿假单胞菌的一种有希望的治疗选择。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian Journal of Microbiology MICROBIOLOGY-

CiteScore

2.40

自引率

7.10%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.